Improved production of jiangxienone in submerged fermentation of Cordyceps jiangxiensis under nitrogen deficiency
Improved production of jiangxienone in submerged fermentation of Cordyceps jiangxiensis under nitrogen deficiency
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缺氮条件下江西虫草深层发酵提高江西烯酮产量
DOI：
10.1007/s00449-018-1970-8
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发表时间：
2018-06
影响因子：
3.8
通讯作者：
Xiao Jian-Hui
中科院分区：
文献类型：
--
作者：
Jiang Lu-Xi;Han Li-Liang;Wang Hui-Ping;Xu Jun-Wei;Xiao Jian-Hui
冬虫夏草蒋毒酮对多种人类癌细胞，尤其是胃癌细胞具有显著的细胞毒性和良好的选择性。本试验研究了缺氮对江西菇深层发酵中姜香酮积累和姜香酮生物合成基因转录水平的影响。结果表明，在缺氮条件下，姜血酮的积累加快。当谷氨酰胺浓度为5 mM时，降血酮含量最高，为3.2µg/g细胞干重，是对照组（60 mM）的8.9倍。在5 mM谷氨酰胺的培养下，生物合成途径基因hmgr和sqs以及氮调控基因areA的转录水平分别比对照上调了7倍、14倍和28倍。推测江溪草的降血酮生物合成可能涉及甲羟戊酸途径。综上所述，本研究表明缺氮是提高姜酮在姜酮发酵过程中积累的有效策略，有助于进一步了解姜酮的生物合成调控。
Jiangxienone produced by Cordyceps jiangxiensis exhibits significant cytotoxicity and good selectivity against various human cancer cells, especially gastric cancer cells. In this work, the effect of nitrogen deficiency on the accumulation of jiangxienone and the transcription levels of jiangxienone biosynthesis genes was studied in submerged fermentation of C. jiangxiensis. Results showed that accumulation of jiangxienone was improved under nitrogen deficiency condition. A maximal jiangxienone content of 3.2 µg/g cell dry weight was reached at 5 mM glutamine, and it was about 8.9-fold higher than that obtained at 60 mM glutamine (control). The transcription levels of the biosynthetic pathway genes hmgr and sqs and the nitrogen regulatory gene areA were upregulated by 7-, 14-, and 28-fold, respectively, in culture with 5 mM glutamine compared to the control. It was hypothesized that the jiangxienone biosynthesis may involve the mevalonate pathway in C. jiangxiensis. Taken together, our study indicated that nitrogen deficiency is an efficient strategy for enhancing jiangxienone accumulation in submerged fermentation of C. jiangxiensis, which is useful for further understanding the regulation of jiangxienone biosynthesis.
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影响因子：
3.8
作者：
Xiangqun Xu;Yan Hu;Lili Quan
通讯作者：
Xiangqun Xu;Yan Hu;Lili Quan
影响因子：
3.7
作者：
Brzonkalik K;Hümmer D;Syldatk C;Neumann A
通讯作者：
Neumann A
DOI：
10.1016/j.ijbiomac.2016.08.002
发表时间：
2016-11-01
影响因子：
8.2
作者：
Lin, Shan;Liu, Zhi-Qiang;Zheng, Yu-Guo
通讯作者：
Zheng, Yu-Guo
影响因子：
--
作者：
Liu, ZY;Liang, ZQ;Yu, ZN
通讯作者：
Yu, ZN
DOI：
10.1016/j.ijbiomac.2015.04.040
发表时间：
2015-08-01
影响因子：
8.2
作者：
Wang, Lan-Ying;Cheong, Kit-Leong;Li, Shao-Ping
通讯作者：
Li, Shao-Ping