Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA.
Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA.
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使用类型特异性 ELISA 对日本马疱疹病毒 1 型和 4 型感染进行诊断和血清流行病学。
DOI：
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发表时间：
1998
影响因子：
1.2
通讯作者：
T. Inoue
中科院分区：
文献类型：
--
作者：
S. Yasunaga;K. Maeda;T. Matsumura;K. Kai;H. Iwata;T. Inoue
最近，Crabb和Studdert[1993]开发了一种使用马疱疹病毒1型（EHV-1）和4型（EHV-4）糖蛋白Gs （gGs）的类型特异性ELISA。为了调查EHV-1和-4在日本马中的传播情况，我们采用了适用于血清学区分EHV-1和-4感染的ELISA方法。通过对感染EHV-1或-4的马的配对血清，证实了ELISA的型别特异性。采用ELISA法对1995-1996年冬季前后采集的80匹赛马血清进行检测，结果显示，30匹马对EHV-1有显著抗体应答，9匹马对EHV-4有显著抗体应答。结果表明，该酶联免疫吸附试验可用于ehev -1和-4感染的诊断和流行病学研究。
Recently, a type-specific ELISA using equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4) glycoprotein Gs (gGs) was developed by Crabb and Studdert [1993]. To investigate the dissemination of EHV-1 and -4 among horses in Japan, we applied their ELISA as suitable for discriminating between EHV-1 and -4 infections serologically. Type-specificity of the ELISA was confirmed by using paired sera of infected horses with either EHV-1 or -4. Application of the ELISA to sera collected before and after the winter season of 1995-1996 from 80 racehorses revealed that 30 horses showed significant antibody responses against EHV-1 and 9 against EHV-4, respectively. The results indicated that this ELISA using paired sera is useful for a diagnosis and an epizootiological study on EHV-1 and -4 infections.
通过限制性核酸内切酶区分马疱疹病毒 1 的呼吸道和致流产分离株。
DOI：
10.1126/science.6270790
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Studdert,MJ;Simpson,T;Roizman,B
通讯作者：
Roizman,B