Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru.
Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru.
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DOI：
10.1038/srep02797
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发表时间：
2013-09-30
影响因子：
4.6
通讯作者：
Udhayakumar, Venkatachalam
中科院分区：
文献类型：
--
作者：
Akinyi, Sheila;Hayden, Tonya;Gamboa, Dionicia;Torres, Katherine;Bendezu, Jorge;Abdallah, Joseph F.;Griffing, Sean M.;Marquino Quezada, Wilmer;Arrospide, Nancy;De Oliveira, Alexandre Macedo;Lucas, Carmen;Magill, Alan J.;Bacon, David J.;Barnwell, John W.;Udhayakumar, Venkatachalam
大多数疟疾快速诊断试验(RDT)检测到由pfhrp2基因编码的恶性疟原虫富含组氨酸蛋白2(PfHRP2)。最近，来自秘鲁的恶性疟原虫分离株被发现缺乏pfhrp2，导致RDT结果假阴性。我们假设，在秘鲁，pfhrp2缺失的寄生虫源自单一的遗传事件。我们分别使用7个中性和7个8号染色体微卫星标记对1998-2005年间收集的样本的寄生虫种群结构和pfhrp2单倍型进行了评估。在1998-2001年间，检测到5种不同的pfhrp2单倍型，对应于5个中性微卫星克隆谱系；pfhrp2缺失发生在4个单倍型中。在2003-2005年间，寄生虫谱系之间的异交导致8个种群集群继承了以前看到的5个pfhrp2单倍型和一个新的单倍型；其中4个单倍型发生了pfhrp2缺失。这些发现表明，在秘鲁，pfhrp2缺失的遗传起源不是单一事件，而可能是多次发生。
The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.
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影响因子：
64.5
作者：
POLOGE, LG;RAVETCH, JV
通讯作者：
RAVETCH, JV
影响因子：
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作者：
Gamboa D;Ho MF;Bendezu J;Torres K;Chiodini PL;Barnwell JW;Incardona S;Perkins M;Bell D;McCarthy J;Cheng Q
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Udhayakumar, Venkatachalam
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通讯作者：
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