Quality assurance of metabolomics.
Quality assurance of metabolomics.
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DOI：
10.14573/altex.1509161
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hartung T
中科院分区：
文献类型：
--
作者：
Bouhifd M;Beger R;Flynn T;Guo L;Harris G;Hogberg H;Kaddurah-Daouk R;Kamp H;Kleensang A;Maertens A;Odwin-DaCosta S;Pamies D;Robertson D;Smirnova L;Sun J;Zhao L;Hartung T
代谢组学承诺一个整体的表型表征的生物反应毒物。该技术基于具有合理吞吐量的先进化学分析工具，包括质谱和NMR。然而，从实验设计、样品制备、代谢物鉴定到生物信息学数据挖掘，迫切需要质量保证，以确保代谢组学数据的质量和生物模型的再现性。与基于微阵列的转录组学相比，在过去的二十年中，已经形成了关于质量保证和报告标准的共识，代谢组学的质量保证现在才出现。安全科学的规范使用，甚至这些技术的正确科学使用，都需要质量保证。为了促进这一讨论，一个专家讲习班讨论了代谢组学的质量保证需求。本次研讨会的目标是：1）考虑与代谢组学作为一门新兴科学相关的挑战，重点是其在毒理学中的应用; 2）确定需要解决的关键问题，以建立和实施基于代谢组学的毒理学质量保证程序。在最佳做法方面仍需达成共识，以确保从这些新工具中获得合理、有用和相关的信息。
Metabolomics promises a holistic phenotypic characterization of biological responses to toxicants. This technology is based on advanced chemical analytical tools with reasonable throughput, including mass-spectroscopy and NMR. Quality assurance, however – from experimental design, sample preparation, metabolite identification, to bioinformatics data-mining – is urgently needed to assure both quality of metabolomics data and reproducibility of biological models. In contrast to microarray-based transcriptomics, where consensus on quality assurance and reporting standards has been fostered over the last two decades, quality assurance of metabolomics is only now emerging. Regulatory use in safety sciences, and even proper scientific use of these technologies, demand quality assurance. In an effort to promote this discussion, an expert workshop discussed the quality assurance needs of metabolomics. The goals for this workshop were 1) to consider the challenges associated with metabolomics as an emerging science, with an emphasis on its application in toxicology and 2) to identify the key issues to be addressed in order to establish and implement quality assurance procedures in metabolomics-based toxicology. Consensus has still to be achieved regarding best practices to make sure sound, useful, and relevant information is derived from these new tools.
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影响因子：
4.4
作者：
Craig, Andrew;Sidaway, James;Nicholson, Jeremy
通讯作者：
Nicholson, Jeremy
影响因子：
5.6
作者：
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通讯作者：
Thomas, Russell
影响因子：
13.1
作者：
Dunn, WB;Ellis, DI
通讯作者：
Ellis, DI
DOI：
10.1016/j.ejpb.2010.12.027
发表时间：
2011-04-01
影响因子：
4.9
作者：
Hartung, Thomas
通讯作者：
Hartung, Thomas
影响因子：
5.6
作者：
Kleensang, Andre;Maertens, Alexandra;Hartung, Thomas
通讯作者：
Hartung, Thomas