Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury
Loc680254 regulates Schwann cell proliferation through Psrc1 and Ska1 as a microRNA sponge following sciatic nerve injury
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Loc680254 在坐骨神经损伤后作为 microRNA 海绵通过 Psrc1 和 Ska1 调节雪旺细胞增殖
作者：
Chun Yao;Qihui Wang;Yaxian Wang;Jiancheng Wu;Xuemin Cao;Yan Lu;Yanping Chen;Wei Feng;Xiaosong Gu;Xin‐Peng Dun;Bin Yu
Peripheral nerve injury triggers sequential phenotype alterations in Schwann cells, which are critical for axonal regeneration. Long noncoding RNAs (lncRNAs) are long transcripts without obvious coding potential. It has been reported that lncRNAs participate in diverse biological processes and diseases. However, the role of lncRNA in Schwann cells and peripheral nerve regeneration is unclear. Here, we identified an lncRNA, loc680254, which is upregulated in rat sciatic nerve after peripheral nerve injury. The loc680254 knockdown inhibits Schwann cell proliferation, enhances apoptosis, and hinders cell cycle, while loc680254 overexpression has the opposite effect. Mechanically, we found that loc680254 might act as a microRNA sponge to regulate the expression of mitosis‐related gene, spindle and kinetochore associated complex subunit 1 (Ska1) and proline/serine‐rich coiled‐coil 1 (Psrc1). Silencing of Psrc1 or Ska1 attenuates the effect of loc680254 overexpression on Schwann cell proliferation. Finally, we repaired the rat sciatic nerve gap with chitosan scaffolds loaded with loc680254‐overexpressing Schwann cells and evaluated axon regeneration and functional recovery. Our results indicated that loc680254 is a new potential modulator for Schwann cell proliferation, which could be targeted to develop novel therapeutic strategies for peripheral nerve repair.
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DOI：
10.1083/jcb.201205025
发表时间：
2012-07-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fontana X;Hristova M;Da Costa C;Patodia S;Thei L;Makwana M;Spencer-Dene B;Latouche M;Mirsky R;Jessen KR;Klein R;Raivich G;Behrens A
通讯作者：
Behrens A
长的非编码RNA通过在原发性传入神经元中沉默的KCNA2导致神经性疼痛。
影响因子：
25
作者：
Zhao, Xiuli;Tang, Zongxiang;Zhang, Hongkang;Atianjoh, Fidelis E.;Zhao, Jian-Yuan;Liang, Lingli;Wang, Wei;Guan, Xiaowei;Kao, Sheng-Chin;Tiwari, Vinod;Gao, Yong-Jing;Hoffman, Paul N.;Cui, Hengmi;Li, Min;Dong, Xinzhong;Tao, Yuan-Xiang
通讯作者：
Tao, Yuan-Xiang
影响因子：
4.4
作者：
Kogenaru S;Qing Y;Guo Y;Wang N
通讯作者：
Wang N
DOI：
--
发表时间：
2023
期刊：
--
影响因子：
--
作者：
Lesia Hiltai
通讯作者：
Lesia Hiltai
影响因子：
4
作者：
Bin Yu;Songlin Zhou;Yongjun Wang;Tianmei Qian;Guohui Ding;F. Ding;X. Gu
通讯作者：
Bin Yu;Songlin Zhou;Yongjun Wang;Tianmei Qian;Guohui Ding;F. Ding;X. Gu