Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force.
Physical limits of cell migration: control by ECM space and nuclear deformation and tuning by proteolysis and traction force.
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DOI：
10.1083/jcb.201210152
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发表时间：
2013-06-24
期刊：
影响因子：
--
通讯作者：
Friedl P
中科院分区：
文献类型：
--
作者：
Wolf K;Te Lindert M;Krause M;Alexander S;Te Riet J;Willis AL;Hoffman RM;Figdor CG;Weiss SJ;Friedl P
The physical limits of cell migration in dense porous environments are dependent upon the available space and the deformability of the nucleus and are modulated by matrix metalloproteinases, integrins and actomyosin function. Cell migration through 3D tissue depends on a physicochemical balance between cell deformability and physical tissue constraints. Migration rates are further governed by the capacity to degrade ECM by proteolytic enzymes, particularly matrix metalloproteinases (MMPs), and integrin- and actomyosin-mediated mechanocoupling. Yet, how these parameters cooperate when space is confined remains unclear. Using MMP-degradable collagen lattices or nondegradable substrates of varying porosity, we quantitatively identify the limits of cell migration by physical arrest. MMP-independent migration declined as linear function of pore size and with deformation of the nucleus, with arrest reached at 10% of the nuclear cross section (tumor cells, 7 µm2; T cells, 4 µm2; neutrophils, 2 µm2). Residual migration under space restriction strongly depended upon MMP-dependent ECM cleavage by enlarging matrix pore diameters, and integrin- and actomyosin-dependent force generation, which jointly propelled the nucleus. The limits of interstitial cell migration thus depend upon scaffold porosity and deformation of the nucleus, with pericellular collagenolysis and mechanocoupling as modulators.
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影响因子：
4.8
作者：
Balzer, Eric M.;Tong, Ziqiu;Konstantopoulos, Konstantinos
通讯作者：
Konstantopoulos, Konstantinos
影响因子：
64.5
作者：
Hotary, KB;Allen, ED;Weiss, SJ
通讯作者：
Weiss, SJ
DOI：
10.1083/jcb.149.6.1309
发表时间：
2000-06-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hotary K;Allen E;Punturieri A;Yana I;Weiss SJ
通讯作者：
Weiss SJ
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4
作者：
Fisher, Kevin E.;Sacharidou, Anastasia;Davis, George E.
通讯作者：
Davis, George E.