Cellular basis for singing motor pattern generation in the field cricket (Gryllus bimaculatus DeGeer).
Cellular basis for singing motor pattern generation in the field cricket (Gryllus bimaculatus DeGeer).
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DOI：
10.1002/brb3.89
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发表时间：
2012-11
影响因子：
3.1
通讯作者：
Hedwig, Berthold
中科院分区：
文献类型：
--
作者：
Schoneich, Stefan;Hedwig, Berthold
关键词：
雄性蟋蟀的鸣叫行为允许分析由性选择塑造的中央模式发生器（CPG），以可靠地产生物种特异性通信信号。在定位了双斑蟋蟀歌唱的基本神经节后，我们现在在细胞水平上研究了叫声CPG。虚构的歌唱是由药物脑刺激启动的。运动模式的音节和啁啾被记录为交替尖峰爆发的翅膀打开和翅膀关闭运动神经元在截断的翅膀神经，它准确地反映了自然的呼叫歌曲。在虚构的歌唱，我们在细胞内记录和染色的中间神经元在胸和腹神经节，并测试其影响的歌曲模式的细胞内电流注入。我们确定了三个interneurons的后胸和第一个未融合的腹部神经节，有节奏地去极化和超极化的阶段与音节模式和严格的前翼开放运动神经元飙升。去极化电流注入两个开放的中间神经元引起额外的节奏性歌唱活动，可靠地重置正在进行的啁啾节奏。歌唱中间神经元的紧密交织的分支揭示了背中线神经桩的后胸和三个最前面的腹部神经节作为歌唱模式生成的解剖位置。在相同的神经桩，我们还记录了几个更密切的中间神经元，有节奏地超极化和去极化的音节节奏和严格的尖峰前翼更接近运动神经元。其中一些在每次啁啾开始时受到明显的抑制。超极化电流注入树突揭示了抑制后反弹去极化作为一个功能机制的中央模式产生在唱歌蟋蟀。
The singing behavior of male crickets allows analyzing a central pattern generator (CPG) that was shaped by sexual selection for reliable production of species-specific communication signals. After localizing the essential ganglia for singing in Gryllus bimaculatus, we now studied the calling song CPG at the cellular level. Fictive singing was initiated by pharmacological brain stimulation. The motor pattern underlying syllables and chirps was recorded as alternating spike bursts of wing-opener and wing-closer motoneurons in a truncated wing nerve; it precisely reflected the natural calling song. During fictive singing, we intracellularly recorded and stained interneurons in thoracic and abdominal ganglia and tested their impact on the song pattern by intracellular current injections. We identified three interneurons of the metathoracic and first unfused abdominal ganglion that rhythmically de- and hyperpolarized in phase with the syllable pattern and spiked strictly before the wing-opener motoneurons. Depolarizing current injection in two of these opener interneurons caused additional rhythmic singing activity, which reliably reset the ongoing chirp rhythm. The closely intermeshing arborizations of the singing interneurons revealed the dorsal midline neuropiles of the metathoracic and three most anterior abdominal neuromeres as the anatomical location of singing pattern generation. In the same neuropiles, we also recorded several closer interneurons that rhythmically hyper- and depolarized in the syllable rhythm and spiked strictly before the wing-closer motoneurons. Some of them received pronounced inhibition at the beginning of each chirp. Hyperpolarizing current injection in the dendrite revealed postinhibitory rebound depolarization as one functional mechanism of central pattern generation in singing crickets.
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影响因子：
3.3
作者：
ALEXANDER, RD
通讯作者：
ALEXANDER, RD
影响因子：
56.9
作者：
DELCOMYN, F
通讯作者：
DELCOMYN, F
影响因子：
56.9
作者：
DUMONT, JPC;ROBERTSON, RM
通讯作者：
ROBERTSON, RM
影响因子：
3.3
作者：
Grace, Jaime L.;Shaw, Kerry L.
通讯作者：
Shaw, Kerry L.
影响因子：
2.5
作者：
Hedwig, B
通讯作者：
Hedwig, B