KIF13A motors are regulated by Rab22A to function as weak dimers inside the cell.
KIF13A motors are regulated by Rab22A to function as weak dimers inside the cell.
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KIF13A电动机由RAB22A调节，以充当细胞内部的弱二聚体。
DOI：
10.1126/sciadv.abd2054
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发表时间：
2021-03
期刊：
影响因子：
13.6
通讯作者：
Soppina V
中科院分区：
文献类型：
--
作者：
Patel NM;Siva MSA;Kumari R;Shewale DJ;Rai A;Ritt M;Sharma P;Setty SRG;Sivaramakrishnan S;Soppina V
KIF13A functions as a weak dimer, critical for recycling endosomal tubulation in vivo. Endocytic recycling is a complex itinerary, critical for many cellular processes. Membrane tubulation is a hallmark of recycling endosomes (REs), mediated by KIF13A, a kinesin-3 family motor. Understanding the regulatory mechanism of KIF13A in RE tubulation and cargo recycling is of fundamental importance but is overlooked. Here, we report a unique mechanism of KIF13A dimerization modulated by Rab22A, a small guanosine triphosphatase, during RE tubulation. A conserved proline between neck coil–coiled-coil (NC-CC1) domains of KIF13A creates steric hindrance, rendering the motors as inactive monomers. Rab22A plays an unusual role by binding to NC-CC1 domains of KIF13A, relieving proline-mediated inhibition and facilitating motor dimerization. As a result, KIF13A motors produce balanced motility and force against multiple dyneins in a molecular tug-of-war to regulate RE tubulation and homeostasis. Together, our findings demonstrate that KIF13A motors are tuned at a single-molecule level to function as weak dimers on the cellular cargo.
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影响因子：
7.7
作者：
Lee PL;Ohlson MB;Pfeffer SR
通讯作者：
Pfeffer SR
影响因子：
11.8
作者：
Grigoriev, Ilya;Splinter, Daniel;Akhmanova, Anna
通讯作者：
Akhmanova, Anna
影响因子：
4.5
作者：
Mrakovic, Amra;Kay, Jason G.;Botelho, Roberto J.
通讯作者：
Botelho, Roberto J.
影响因子：
1.7
作者：
Li, Xueyi;Sapp, Ellen;DiFiglia, Marian
通讯作者：
DiFiglia, Marian
DOI：
10.1083/jcb.139.2.469
发表时间：
1997-10-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Burkhardt JK;Echeverri CJ;Nilsson T;Vallee RB
通讯作者：
Vallee RB