Polypeptide synthesis using an expressed peptide as a building block for condensation with a peptide thioester: Application to the synthesis of phosphorylated p21Max protein(1–101)
Polypeptide synthesis using an expressed peptide as a building block for condensation with a peptide thioester: Application to the synthesis of phosphorylated p21Max protein(1–101)
复制标题
使用表达肽作为与肽硫酯缩合的结构单元进行多肽合成：在磷酸化 p21Max 蛋白合成中的应用(1–101)
DOI：
10.1002/psc.341
复制
发表时间：
2001
影响因子：
2.1
通讯作者：
S. Aimoto
中科院分区：
文献类型：
--
作者：
T. Kawakami;K. Hasegawa;Kenta Teruya;K. Akaji;M. Horiuchi;F. Inagaki;Y. Kurihara;S. Uesugi;S. Aimoto
一个表达的肽被证明是有用的作为一个构建块通过硫酯方法合成多肽。可以从重组蛋白中制备部分保护的肽段，用作C -末端的构建块；将其N端氨基酸残基转氨化为α‐氧酰基，然后用t‐丁氧羰基（Boc）保护侧链氨基，最后去除α‐氧酰基。另一方面，利用Boc化学通过固相法合成了一种含O -磷酸丝氨酸的肽硫酯。然后这些结构块在银离子和活性酯组分的存在下被浓缩。在缩合过程中，用N，N -二甲基甲酰胺（DMF）作为溶剂可以抑制缩合部位的外映反应。利用这一策略，成功合成了p21Max蛋白的磷酸化部分肽[Ser(PO3H2)2,11]‐p21Max（1-101）。版权所有©2001欧洲多肽协会和约翰威利父子有限公司
An expressed peptide proved to be useful as a building block for the synthesis of a polypeptide via the thioester method. A partially protected peptide segment, for use as a C‐terminal building block, could be prepared from a recombinant protein; its N‐terminal amino acid residue was transaminated to an α‐oxoacyl group, the side‐chain amino groups were then protected with t‐butoxycarbonyl (Boc) groups, and, finally, the α‐oxoacyl group was removed. On the other hand, an O‐phosphoserine‐containing peptide thioester was synthesized via a solid‐phase method using Boc chemistry. These building blocks were then condensed in the presence of silver ions and an active ester component. During the condensation, epimerization at the condensation site could be suppressed by the use of N,N‐dimthylformamide (DMF) as a solvent. Using this strategy, a phosphorylated partial peptide of the p21Max protein, [Ser(PO3H2)2,11]‐p21Max(1–101), was successfully synthesized. Copyright © 2001 European Peptide Society and John Wiley & Sons, Ltd.
登录
查看更多内容
影响因子：
56.9
作者：
DAWSON, PE;MUIR, TW;KENT, SBH
通讯作者：
KENT, SBH
影响因子：
56.9
作者：
BLACKWOOD, EM;EISENMAN, RN
通讯作者：
EISENMAN, RN
DOI：
10.1073/pnas.96.2.388
发表时间：
1999-01-19
影响因子：
11.1
作者：
Xu, R;Ayers, B;Muir, TW
通讯作者：
Muir, TW
影响因子：
56.9
作者：
KANE, PM;YAMASHIRO, CT;STEVENS, TH
通讯作者：
STEVENS, TH