Glycosylated aniline polymer sensor: amine to imine conversion on protein-carbohydrate binding.
Glycosylated aniline polymer sensor: amine to imine conversion on protein-carbohydrate binding.
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DOI：
10.1016/j.bios.2013.02.030
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发表时间：
2013-08-15
影响因子：
12.6
通讯作者：
Zeng, Xiangqun
中科院分区：
文献类型：
--
作者：
Wang, Zhe;Sun, Chunyan;Vegesna, Giri;Liu, Haiying;Liu, Yang;Li, Jinghong;Zeng, Xiangqun
本文以功能化甘露糖基苯胺聚合物(甘露聚苯胺)为电化学平台，通过研究甘露糖胺与蛋白质结合时电导率的变化来研究糖与蛋白质之间的相互作用。用UV-Vis光谱研究了甘露聚苯胺与刀豆蛋白A(ConA)结合过程中聚苯胺含量(从胺到亚胺)的相互转化。X射线光电子能谱(XPS)和紫外-可见光谱(UV-Vis)的结果都表明，ConA与甘露聚苯胺薄膜的结合触发了聚苯胺主链中胺类官能团的转换，将它们转化为亚胺形式。在pH=7.4的缓冲溶液中，通过测量甘露聚苯胺膜的阻抗变化来定量ConA与甘露糖之间的特异性相互作用，得到了ConA的阻抗与ConA浓度之间的线性关系，检出限达到0.12 nM，而在相同的甘露聚苯胺膜中加入非特异性的对照凝集素，阻抗变化很小。对甘露聚苯胺薄膜20周的稳定性表征表明，与原始信号的最大漂移仅为3%。因此，构建独特的碳水化合物-PANI杂交物是一种很有前途的新的碳水化合物识别部分，用于研究碳水化合物-蛋白质相互作用，可能导致一种新的电化学方法来表征碳水化合物-蛋白质相互作用和碳水化合物介导的细胞间识别。
In this report, functionalized mannosylated aniline polymer (manno-PANI) was investigated as an electrochemical platform to study carbohydrate–protein interactions by exploiting the conductivity change of manno-PANI when the specific lectin binding occurs. A systematic study was performed to characterize the interconversion of polyaniline content (from amine to imine) in manno-PANI by UV–vis spectroscopy during its binding with concanavalin A (Con A). Both X-ray photoelectron spectrometry (XPS) and UV–vis results suggest that Con A binding with the manno-PANI film triggers the switching of amine functionalities in the polyaniline backbone, converting them to imine forms. Electrochemical impedance spectroscopy (EIS) was used to quantify the specific interactions between Con A and mannose by measuring the impedance change of manno-PANI film for the detection of Con A. A linear relationship between the impedance and Con A concentration was obtained, and the detection limit reaches to 0.12 nM Con A in a buffer solution (pH=7.4), whereas the addition of nonspecific control lectins to the same manno-PANI film gave very little impedance variations. Stability characterization of the manno-PANI film over 20 weeks shows a maximum drift of only 3% from the original signal. Thus, the uniquely constructed carbohydrate–PANI hybrid is a promising new carbohydrate recognition moiety for studying carbohydrate-protein interactions, presumably leading to a new electrochemical method for characterization of carbohydrate–protein interactions and carbohydrate-mediated intercellular recognitions.
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