Expression and localization of MrgprD in mouse intestinal tract
Expression and localization of MrgprD in mouse intestinal tract
复制标题
MrgprD 在小鼠肠道中的表达和定位
DOI：
10.1007/s00441-019-03017-7
复制
发表时间：
2019-03
影响因子：
3.6
通讯作者：
Lan Lei
中科院分区：
文献类型：
--
作者：
Zhou Chenxing;Li Jia;Liu Lin;Tang Zongxiang;Wan Fengyi;Lan Lei
MrgprD是一种与MAS相关的G蛋白偶联受体，最初在小鼠背根神经节（DRG）的感觉神经元中被鉴定，并已被认为参与躯体感觉。然而，最近发现MrgprD在神经系统外表达，例如在主动脉内皮细胞和中性粒细胞中。本研究采用免疫组织化学方法检测MrgprD在小鼠肠道的表达和定位。MrgprD的免疫反应（IR）在小肠、结肠和直肠的平滑肌层中被发现。此外，MrgprD IR与驻留在肠粘膜固有层中的F4/80阳性巨噬细胞和CD 3阳性T淋巴细胞共定位。MrgprD也被发现在原代腹腔巨噬细胞和脾T淋巴细胞中表达。此外，在小鼠巨噬细胞样RAW 264.7和人T淋巴细胞Jurkat细胞系中检测到MrgprD mRNA及其蛋白的存在。我们的研究首次显示了MrgprD在肠道、巨噬细胞和T淋巴细胞中的表达和定位，表明MrgprD在肠道运动和免疫中的潜在作用。
MrgprD, a Mas-related G protein-coupled receptor, is initially identified in sensory neurons of mouse dorsal root ganglia (DRG) and has been suggested to participate in somatosensation. However, MrgprD has recently been found to be expressed outside the nervous system such as in aortic endothelia cells and neutrophils. In this study, we used immunohistochemistry to detect the expression and localization of MrgprD in mouse intestinal tract. The immunoreactivity (IR) of MrgprD was found in the smooth muscle layers of small intestine, colon and rectum. In addition, MrgprD IR was colocalized with F4/80-positive macrophages and CD3-positive T lymphocytes resident in the lamina propria of intestinal mucosa. MrgprD was also found to be expressed in primary peritoneal macrophages and splenic T lymphocytes. Furthermore, the presence of MrgprD mRNA and its protein was detected in murine macrophage-like RAW 264.7 and human T lymphocyte Jurkat cell lines. Our study shows, for the first time, the expression and localization of MrgprD in the intestinal tract and in macrophages and T lymphocytes, indicating the potential roles of MrgprD in intestinal mobility and immunity.
登录
查看更多内容
DOI：
10.1523/jneurosci.3509-12.2012
发表时间：
2012-10-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Liu Q;Sikand P;Ma C;Tang Z;Han L;Li Z;Sun S;LaMotte RH;Dong X
通讯作者：
Dong X
影响因子：
20.1
作者：
Lautner, Roberto Queiroga;Villela, Daniel C.;Santos, Robson A. S.
通讯作者：
Santos, Robson A. S.
DOI：
10.1073/pnas.1732949100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Zylka, MJ;Dong, XZ;Anderson, DJ
通讯作者：
Anderson, DJ
影响因子：
5.3
作者：
Crozier, Robert A.;Ajit, Seena K.;Pausch, Mark H.
通讯作者：
Pausch, Mark H.
影响因子：
5.5
作者：
Da Silva, Analina R.;Lenglet, Sebastien;Montecucco, Fabrizio
通讯作者：
Montecucco, Fabrizio