Histamine Stimulation of Inositol 1‐Phosphate Accumulation in Lithium‐Treated Slices from Regions of Guinea Pig Brain
Histamine Stimulation of Inositol 1‐Phosphate Accumulation in Lithium‐Treated Slices from Regions of Guinea Pig Brain
复制标题
组胺刺激豚鼠大脑区域经锂处理的切片中肌醇 1-磷酸盐的积累
DOI：
10.1111/j.1471-4159.1984.tb06674.x
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发表时间：
1984
影响因子：
4.7
通讯作者：
J. Young
中科院分区：
文献类型：
--
作者：
P. Daum;C. Downes;J. Young
摘要：在存在10 mM LiCl的情况下，组胺刺激豚鼠脑几个区域的[3 H]肌醇负载组织切片中[3 H]肌醇1-磷酸的蓄积。[3 H]肌醇1-磷酸的水平近似线性增加，经过一个初始的滞后期，最多的时间为120 mm。在没有锂离子的情况下，在大脑皮层和海马切片中组胺刺激的1-磷酸的积累显着减少。锂离子对小脑脑片组胺反应的影响要小得多。对组胺的反应特征与H1受体的介导一致，并且对美托咪胺（2.3 × 109 M −1）和美沙吡林（1.8 × 109 M −1）的亲和力常数与对豚鼠其他H1反应的测量结果相似。组胺的EC 50在小脑、大脑皮层、海马和下丘脑中相似。大脑皮质切片中组胺的剂量反应曲线位置与根据组胺抑制[3 H]美托咪胺结合推导的组胺受体结合曲线位置相似。
Abstract: Histamine stimulated the accumulation of [3H]inositol 1‐phosphate in the presence of 10 mM LiCl in [3H]inositol‐loaded tissue slices from several regions of guinea pig brain. The level of [3H]inositol 1‐phosphate increased approximately linearly, after an initial lag period, up to a time of 120 mm. In the absence of lithium ions the accumulation of the 1‐phosphate stimulated by histamine in cerebral cortical and hippocampal slices was markedly reduced. Lithium ions had much less effect on the response to histamine in cerebellar slices. The characteristics of the response to histamine were consistent with mediation by H1, receptors, and the affinity constants derived for mepyramine (2.3 × 109M−1) and methapyrilene(1.8 × 109M−1) were similar to those reported from measurements on other H1 responses in the guinea pig. The EC50 for histamine was similar in cerebellum, cerebral cortex, hippocampus, and hypothalamus. The position of the dose‐response curve for histamine in cerebral cortical slices was similar to ‐that of the curve for the receptor binding of histamine deduced from histamine inhibition of [3H]mepyramine binding.
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DOI：
10.1042/bj2060555
发表时间：
1982
期刊：
The Biochemical journal
影响因子：
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Weiss,SJ;McKinney,JS;PutneyJr,JW
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DOI：
10.1016/s0021-9258(19)70391-3
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期刊：
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影响因子：
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作者：
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DOI：
10.1073/pnas.78.3.1366
发表时间：
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影响因子：
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通讯作者：
Loeb,JN