Sequence analysis of Salmonella enterica isolates obtained from shelter dogs throughout Texas.
Sequence analysis of Salmonella enterica isolates obtained from shelter dogs throughout Texas.
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对从德克萨斯州各地收容所犬中获得的肠沙门氏菌分离株进行序列分析。
DOI：
10.1002/vms3.320
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发表时间：
2020-11
影响因子：
1.7
通讯作者：
Goodman LB
中科院分区：
文献类型：
--
作者：
Cummings KJ;Mitchell PK;Rodriguez-Rivera LD;Goodman LB
狗是人畜共患沙门氏菌传播的潜在来源。我们以前使用重复横断面研究设计估计了整个德克萨斯州收容所狗中沙门氏菌脱落的流行程度。我们目前的目标是利用全基因组测序来充分表征沙门氏菌分离株并评估它们的亲缘关系。4/27（15%）分离株检测到抗微生物药物耐药性基因。在两个分离株中鉴定出磷霉素耐药基因fosA7；据我们所知，在犬沙门氏菌分离物中没有发表过这种基因的报道。对季铵盐化合物具有抗性的杀菌剂抗性基因qacEdelta1在具有4个AMR基因的分离物中被检测到。最常见的血清型是Newport（6/ 27,22%）和Javiana(4/ 27,15%)，这两种血清型在动物收容所中普遍存在。对于这些血清型，有证据表明沙门氏菌在庇护所环境中传播，也有证据表明沙门氏菌被单独引入庇护所。多个犬沙门氏菌分离株与人类临床分离株密切相关（4个犬分离株在10个snp范围内，6个在20个snp范围内），表明存在共同的病原体群体。针对动物收容所工作人员的教育推广计划将有助于优化沙门氏菌和其他与犬相关的人畜共患病病原体的知识。本研究的目的是利用全基因组测序技术，全面表征从德克萨斯州收容所的狗身上分离出的沙门氏菌，并评估它们的亲缘关系。我们发现了沙门氏菌在避难所环境中传播和沙门氏菌单独引入避难所的证据。一些犬沙门氏菌分离株与人类临床分离株密切相关，表明犬和人类病原体之间存在重叠。
Dogs are a potential source of zoonotic Salmonella transmission. We had previously estimated the prevalence of Salmonella shedding among shelter dogs throughout Texas using a repeated cross‐sectional study design. Our current objectives were to fully characterize the Salmonella isolates and to assess their relatedness, using whole‐genome sequencing. Antimicrobial resistance (AMR) genes were detected in 4/27 (15%) of the isolates. The fosfomycin resistance gene fosA7 was identified in two isolates; to our knowledge, there are no published reports of this gene in canine Salmonella isolates. The biocide resistance gene qacEdelta1, conferring resistance to quaternary ammonium compounds, was detected in an isolate that had four additional AMR genes. The most frequently identified serotypes were Newport (6/27, 22%) and Javiana (4/27, 15%), both of which were widespread among animal shelters. For these serotypes, there was evidence of both transmission of Salmonella within the shelter environment and separate introductions of Salmonella into a shelter. Several canine Salmonella isolates were closely related to human clinical isolates (four canine isolates within 10 SNPs and six more within 20 SNPs), suggesting a shared pathogen population. Educational outreach programmes targeting animal shelter workers would be useful for optimizing knowledge of Salmonella and other canine‐associated zoonotic pathogens. The objectives of this study were to fully characterize Salmonella isolates obtained from shelter dogs throughout Texas and to assess their relatedness, using whole‐genome sequencing. We found evidence of both transmission of Salmonella within the shelter environment and separate introductions of Salmonella into a shelter. Several canine Salmonella isolates were closely related to human clinical isolates, demonstrating the overlap between canine and human pathogens.
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影响因子：
4.2
作者：
Clarkson, L. S.;Tobin-D'Angelo, M.;Voetsch, A. C.
通讯作者：
Voetsch, A. C.
影响因子：
2.4
作者：
Leonard, E. K.;Pearl, D. L.;Weese, J. S.
通讯作者：
Weese, J. S.
影响因子：
11.8
作者：
Scallan, Elaine;Griffin, Patricia M.;Hoekstra, Robert M.
通讯作者：
Hoekstra, Robert M.
影响因子：
14.9
作者：
Letunic, Ivica;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
2
作者：
Hoffmann, Sandra;Batz, Michael B.;Morris, J. Glenn, Jr.
通讯作者：
Morris, J. Glenn, Jr.