Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA.
Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA.
复制标题
使用共价闭合环状质粒 DNA 在大肠杆菌 K-12 中进行基因组替换。
DOI:
10.1016/0378-1119(90)90337-q
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发表时间:
1990
期刊:
影响因子:
3.5
通讯作者:
Gennis,RB
中科院分区:
文献类型:
--
作者:
Oden,KL;DeVeaux,LC;Vibat,CR;CronanJr,JE;Gennis,RB
A number of gene replacements at different loci were constructed using covalently closed circular (ccc) plasmid DNA in herecB21recC22sbcB15asbcC201mutant ofEscherichia coli(JC7623). Selected constructs representing deletions and insertion mutations formed from double-crossover events involving the ccc plamid molecules and the genome were confirmed by Southern blots, and the frequency of double-crossover events was evaluated. It is reported that such mutants may be constructed without linearizing plasmid DNA, as described previously.
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DOI:
10.1073/pnas.83.15.5558
发表时间:
1986-08-01
影响因子:
11.1
作者:
AMUNDSEN, SK;TAYLOR, AF;SMITH, GR
通讯作者:
SMITH, GR
影响因子:
14.9
作者:
E. C. Conley;V. Saunders;J. Saunders
通讯作者:
J. Saunders
DOI:
--
发表时间:
1984
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Green,GN;Kranz,RG;Lorence,RM;Gennis,RB
通讯作者:
Gennis,RB
影响因子:
3.2
作者:
W. Hoekstra;J. Bergmans;E. Zuidweg
通讯作者:
E. Zuidweg
影响因子:
14.9
作者:
E. C. Conley;V. Saunders;V. Jackson;J. Saunders
通讯作者:
E. C. Conley;V. Saunders;V. Jackson;J. Saunders