Serum microRNA-210 as a potential noninvasive biomarker for the diagnosis and prognosis of glioma.
Serum microRNA-210 as a potential noninvasive biomarker for the diagnosis and prognosis of glioma.
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DOI：
10.1038/bjc.2015.91
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发表时间：
2015-03-31
影响因子：
8.8
通讯作者：
Xu, S-s
中科院分区：
文献类型：
--
作者：
Lai, N-s;Wu, D-g;Fang, X-g;Lin, Y-c;Chen, S-s;Li, Z-b;Xu, S-s
microRNA-210（miR-210）是一种致癌的miRNA，以前与人类神经胶质瘤（一种无法治愈的中枢神经系统肿瘤类型）的预后相关。在此，miR-210被研究作为胶质瘤诊断和预后的潜在血清生物标志物。从2007年2月至2014年3月在皖南医学院第一附属医院神经外科（中国芜湖）初步诊断时的I-IV级胶质瘤患者（n=136）和健康对照（n=50）采集的血液样品立即制备血清。从血清中分离总RNA。用miR-210和miR-16-1特异性引物（内部对照）合成cDNA，并进行定量实时RT-PCR。对结果进行统计学分析，以确定miR-210在人脑胶质瘤患者诊断和预后中的作用。在胶质母细胞瘤患者的血清样本中检测到miR-210表达相对于健康对照增加约7倍。从受试者操作特征曲线（ROC）中选择阈值表达值（2.259），并通过多变量考克斯比例风险回归和Kaplan-Meier分析对低和高miR-210表达组进行分析。结果显示高血清miR-210表达与肿瘤分级和不良患者结局相关（P值<0.001）。血清miR-210是一种很有前途的诊断和预后生物标志物，可以在胶质瘤患者的外周血中检测到。
MicroRNA-210 (miR-210) is an oncogenic miRNA previously associated with prognosis in human gliomas, an incurable tumour type of the central nervous system. Here miR-210 was investigated as a potential serum biomarker in the diagnosis and prognosis of glioma. Serum was immediately prepared from blood samples collected from patients with glioma grades I–IV at primary diagnosis (n=136) and healthy controls (n=50) from February 2007 to March 2014 in the Department of Neurosurgery of the First Affiliated Hospital of Wannan Medical College (Wuhu, China). Total RNA was isolated from serum. cDNA was synthesised with primers specific for miR-210 and miR-16-1 (internal control), and quantitative real-time RT-PCR was performed. Results were statistically analysed to determine the role of miR-210 in the diagnosis and prognosis of human glioma patients. An approximately seven-fold increase in miR-210 expression was detected in serum samples from glioblastoma patients relative to healthy controls. A threshold expression value (2.259) was chosen from receiver operator characteristic curves (ROC), and the low and high miR-210 expression groups were analysed by multivariate Cox proportional hazard regression and Kaplan–Meier analyses. Results revealed an association of high serum miR-210 expression with tumour grade and poor patient outcome (P-values <0.001). Serum miR-210 is a promising diagnostic and prognostic biomarker that can be detected in the peripheral blood of patients with glioma.
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影响因子：
4.4
作者：
Pizzini S;Bisognin A;Mandruzzato S;Biasiolo M;Facciolli A;Perilli L;Rossi E;Esposito G;Rugge M;Pilati P;Mocellin S;Nitti D;Bortoluzzi S;Zanovello P
通讯作者：
Zanovello P
DOI：
10.1007/s13277-014-1638-1
发表时间：
2014-05
期刊：
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
影响因子：
--
作者：
Srivastava A;Goldberger H;Dimtchev A;Marian C;Soldin O;Li X;Collins SP;Suy S;Kumar D
通讯作者：
Kumar D
影响因子：
--
作者：
Ivan M;Huang X
通讯作者：
Huang X
影响因子：
5.2
作者：
Li, Li'an;Huang, Ke;Meng, Yuanguang
通讯作者：
Meng, Yuanguang
影响因子：
8
作者：
Chen, Z.;Li, Y.;Luthra, R.
通讯作者：
Luthra, R.