Integrating next-generation sequencing and traditional tongue diagnosis to determine tongue coating microbiome.
Integrating next-generation sequencing and traditional tongue diagnosis to determine tongue coating microbiome.
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DOI：
10.1038/srep00936
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发表时间：
2012
影响因子：
4.6
通讯作者：
Li, Shao
中科院分区：
文献类型：
--
作者：
Jiang, Bai;Liang, Xujun;Chen, Yang;Ma, Tao;Liu, Liyang;Li, Junfeng;Jiang, Rui;Chen, Ting;Zhang, Xuegong;Li, Shao
舌诊是中医中一种独特的诊断法。这是首次使用新一代测序技术研究传统舌头诊断与舌苔微生物组之间的关系。该研究包括19名具有典型的白油腻或黄致密舌苔的胃炎患者，分别对应中医寒证或热证，以及8名健康志愿者。设计了Illumina配对端双条形码16S rRNA测序方案来分析舌膜微生物组，从中获得每个样品约370万个V6标签。我们鉴定出123个和258个物种水平的otu分别在寒/热证患者中富集，分别代表“冷微生物群”和“热微生物群”。我们进一步构建了与冷/热证相关的舌头微生物群失衡网络。这些结果揭示了舌苔微生物组与传统的舌头诊断之间的重要联系，并说明了舌苔微生物组作为表征患者亚型的新型整体生物标志物的潜力。
Tongue diagnosis is a unique method in traditional Chinese medicine (TCM). This is the first investigation on the association between traditional tongue diagnosis and the tongue coating microbiome using next-generation sequencing. The study included 19 gastritis patients with a typical white-greasy or yellow-dense tongue coating corresponding to TCM Cold or Hot Syndrome respectively, as well as eight healthy volunteers. An Illumina paired-end, double-barcode 16S rRNA sequencing protocol was designed to profile the tongue-coating microbiome, from which approximately 3.7 million V6 tags for each sample were obtained. We identified 123 and 258 species-level OTUs that were enriched in patients with Cold/Hot Syndromes, respectively, representing "Cold Microbiota" and "Hot Microbiota". We further constructed the tongue microbiota-imbalanced networks associated with Cold/Hot Syndromes. The results reveal an important connection between the tongue-coating microbiome and traditional tongue diagnosis, and illustrate the potential of the tongue-coating microbiome as a novel holistic biomarker for characterizing patient subtypes.
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