An in vitro injury model for SH-SY5Y neuroblastoma cells: Effect of strain and strain rate
An in vitro injury model for SH-SY5Y neuroblastoma cells: Effect of strain and strain rate
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SH-SY5Y 神经母细胞瘤细胞的体外损伤模型：应变和应变率的影响
DOI：
10.1016/j.jneumeth.2012.01.001
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发表时间：
2012
影响因子：
3
通讯作者：
N. Chandra
中科院分区：
文献类型：
--
作者：
M. Skotak;F. Wang;N. Chandra
非常需要体外细胞损伤模型，其中可以精确且独立地应用宽范围的应变（ε）和应变率（ε stec）。这样的模型将能够探索细胞在钝性或爆炸冲击诱导的创伤性脑损伤（TBI）期间通常遇到的各种生物力学载荷条件。结合高度自动化的数据采集和分析系统，该方法可以快速生成大量的实验结果数据集，以识别损伤后的生物力学和化学后遗症。对这些后遗症的正确理解将有助于发现药物干预的时间窗口。在这项研究中，我们提出了这样一种损伤模型，一种改良版的培养轴突损伤（CAI）装置，并通过SH-SY 5 Y细胞在不同应变范围（0-140%）和应变率（15- 68 s-1）下的活力证明了其有效性。我们在SH-SY 5 Y细胞的拉伸诱导剂量反应曲线中确定了三种不同的制度，在狭窄的应变范围内（30-55%）从活细胞到死细胞有非常急剧的下降。当细胞中的最终应变固定在50%时，应变速率的影响最小。该模型进一步表明，损伤后的时间起着至关重要的作用，在确定恢复恶化途径和生物选择取决于初始损伤的严重程度。这些数据指出，初始应变水平对细胞命运至关重要，并强调需要研究不同程度的初始损伤引发的各种机制。
There is a great need to have in vitro cell injury model wherein a wide range of strain (ɛ) and strain rate (ε˙) can be precisely and independently applied. Such a model will enable exploration of various biomechanical loading conditions cells normally encounter during either blunt or blast impact-induced traumatic brain injuries (TBIs). In combination with a highly automated data acquisition and analysis system, this method can quickly generate a large data set of experimental results to yield identification of bio-mechanical and chemical sequelae following injury. A proper understanding of these sequelae will enable the discovery of the time window of opportunity available for pharmacological interventions. In this study we present such an injury model, a modified version of the Cultured Axonal Injury (CAI) device, and demonstrate its efficacy through viability of SH-SY5Y cells at different ranges of strain (0–140%) and strain rate (15–68s−1). We identified three different regimes in the stretch-induced dose–response of curves of SH-SY5Y cells, with a very sharp decline from live to dead in a narrow range of strain (30–55%). The effect of strain rate is minimal when the final strain in the cells was fixed at 50%. The model further shows that time-after-injury plays a vital role in the determination of recovery-deterioration pathways and the biological selection depends on the severity of initial injury. These data point out the initial strain level is vital to the cell fate and emphasize the need to study the various mechanisms triggered by different magnitudes of initial injuries.
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影响因子：
4.2
作者：
Simon, Crystal M.;Sharif, Shan;LaPlaca, Michelle C.
通讯作者：
LaPlaca, Michelle C.
影响因子：
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作者：
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影响因子：
4.2
作者：
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影响因子：
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作者：
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通讯作者：
Hayes, RL
影响因子：
3.3
作者：
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通讯作者：
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