Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium.
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DOI：
10.1083/jcb.201010127
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发表时间：
2011-03-07
期刊：
影响因子：
--
通讯作者：
Jay P
中科院分区：
文献类型：
--
作者：
Gerbe F;van Es JH;Makrini L;Brulin B;Mellitzer G;Robine S;Romagnolo B;Shroyer NF;Bourgaux JF;Pignodel C;Clevers H;Jay P
Tuft cells represent a fourth type of intestinal secretory cell that constitutes the primary source of endogenous intestinal opioids and are the only epithelial cell that constitutively express cyclooxygenases. The unique morphology of tuft cells was first revealed by electron microscopy analyses in several endoderm-derived epithelia. Here, we explore the relationship of these cells with the other cell types of the intestinal epithelium and describe the first marker signature allowing their unambiguous identification. We demonstrate that although mature tuft cells express DCLK1, a putative marker of quiescent stem cells, they are post-mitotic, short lived, derive from Lgr5-expressing epithelial stem cells, and are found in mouse and human tumors. We show that whereas the ATOH1/MATH1 transcription factor is essential for their differentiation, Neurog3, SOX9, GFI1, and SPDEF are dispensable, which distinguishes these cells from enteroendocrine, Paneth, and goblet cells, and raises from three to four the number of secretory cell types in the intestinal epithelium. Moreover, we show that tuft cells are the main source of endogenous intestinal opioids and are the only epithelial cells that express cyclooxygenase enzymes, suggesting important roles for these cells in the intestinal epithelium physiopathology.
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DOI：
10.1083/jcb.200311021
发表时间：
2004-07-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blache P;van de Wetering M;Duluc I;Domon C;Berta P;Freund JN;Clevers H;Jay P
通讯作者：
Jay P
影响因子：
2.5
作者：
Bezencon, C.;Fuerholz, A.;Damak, S.
通讯作者：
Damak, S.
影响因子：
11.4
作者：
Jenny, M;Uhl, C;Gradwohl, G
通讯作者：
Gradwohl, G
影响因子：
5
作者：
Colnot, S;Niwa-Kawakita, M;Perret, C
通讯作者：
Perret, C
DOI：
10.1073/pnas.0706890104
发表时间：
2007-09-18
影响因子：
11.1
作者：
Jang, Hyeung-Jin;Kokrashvili, Zaza;Egan, Josephine M.
通讯作者：
Egan, Josephine M.