Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein.
Nuclear localization of the G protein beta 5/R7-regulator of G protein signaling protein complex is dependent on R7 binding protein.
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DOI：
10.1111/j.1471-4159.2010.06616.x
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发表时间：
2010-06
影响因子：
4.7
通讯作者：
Simonds WF
中科院分区：
文献类型：
--
作者：
Panicker LM;Zhang JH;Posokhova E;Gastinger MJ;Martemyanov KA;Simonds WF
The neuronally expressed Gβ5 subunit is the most structurally divergent among heterotrimeric Gβ isoforms and unique in its ability to heterodimerize with the R7 subfamily of regulator of G protein signaling (RGS) proteins. The complex between Gβ5 and R7-type RGS proteins targets the cell nucleus by an unknown mechanism. Although the nuclear targeting of the Gβ5/R7-RGS complex is proposed to involve the binding of R7-binding protein (R7BP), this theory is challenged by the observations that endogenous R7BP is palmitoylated, co-localizes strongly with the plasma membrane, and has never been identified in the cytosol or nucleus of native neurons or untreated cultured cells. We show here mutant RGS7 lacking the N-terminal Disheveled, EGL-10, Pleckstrin homology (DEP) domain is expressed in transfected cells but, unlike wild type RGS7, is excluded from the cell nucleus. Because the DEP domain is essential for R7BP binding to RGS7, we studied the subcellular localization of Gβ5 in primary neurons and brain from mice deficient in R7BP. The level of endogenous nuclear Gβ5 and RGS7 in neurons and brains from R7BP KO mice is reduced by 50 to 70%. These results suggest that R7BP contributes significantly to the nuclear localization of endogenous Gβ5/R7-RGS complex in brain.
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影响因子：
4.7
作者：
Mancuso JJ;Qian Y;Long C;Wu GY;Wensel TG
通讯作者：
Wensel TG
DOI：
10.1523/jneurosci.1367-09.2009
发表时间：
2009-07-22
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cao Y;Masuho I;Okawa H;Xie K;Asami J;Kammermeier PJ;Maddox DM;Furukawa T;Inoue T;Sampath AP;Martemyanov KA
通讯作者：
Martemyanov KA
影响因子：
5.3
作者：
Anderson, Garret R.;Lujan, Rafael;Martemyanov, Kirill A.
通讯作者：
Martemyanov, Kirill A.
DOI：
10.1016/j.bbamcr.2004.01.005
发表时间：
2004-05-03
影响因子：
5.1
作者：
Bouhamdan, M;Michelhaugh, SK;Bannon, MJ
通讯作者：
Bannon, MJ
影响因子：
3.5
作者：
Song, Joseph H.;Song, Hongman;Martemyanov, Kirill A.
通讯作者：
Martemyanov, Kirill A.