The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability
The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability
复制标题
分子伴侣 Hsp90 通过调节微管稳定性维持高尔基体组织和囊泡运输
DOI：
10.1093/jmcb/mjz093
复制
发表时间：
2019-09
影响因子：
5.5
通讯作者：
Kan Liao
中科院分区：
文献类型：
--
作者：
Yuan;Yubo Ding;Xiudan Zheng;Kan Liao
摘要Hsp90是一种丰富而独特的分子伴侣，被认为是许多转录因子和信号转导酶的调节因子。它的高丰度表明它参与了一些更基本的过程。在这项研究中，我们提供了证据，证明Hsp90是微管稳定、高尔基组织和囊泡运输所必需的。我们发现Hsp90与微管相关蛋白4(MAP4)结合，MAP4是维持微管乙酰化和稳定所必需的。HSP90的缺失导致MAP4的减少，导致微管去乙酰化和不稳定。此外，在Hsp90缺失的细胞中，高尔基体碎裂，顺行囊泡运输受损，其表型与沉默MAP4诱导的表型相似。外源性MAP4的表达或曲古抑素A的治疗可以挽救Hsp90缺失的这些干扰效应，从而增加微管的乙酰化和稳定性。因此，微管稳定性是Hsp90调控的重要细胞事件。
Abstract Hsp90 is an abundant and special molecular chaperone considered to be the regulator of many transcription factors and signaling kinases. Its high abundance is indicative of its involvement in some more fundamental processes. In this study, we provide evidence that Hsp90 is required for microtubule stabilization, Golgi organization, and vesicular trafficking. We showed that Hsp90 is bound to microtubule-associated protein 4 (MAP4), which is essential for maintaining microtubule acetylation and stabilization. Hsp90 depletion led to the decrease in MAP4, causing microtubule deacetylation and destabilization. Furthermore, in Hsp90-depleted cells, the Golgi apparatus was fragmented and anterograde vesicle trafficking was impaired, with phenotypes similar to those induced by silencing MAP4. These disruptive effects of Hsp90 depletion could be rescued by the expression of exogenous MAP4 or the treatment of trichostatin A that increases microtubule acetylation as well as stability. Thus, microtubule stability is an essential cellular event regulated by Hsp90.
登录
查看更多内容
分子伴侣 Hsp90α 缺陷通过破坏光感受器中的高尔基组织和囊泡运输而导致视网膜变性
影响因子：
5.5
作者：
Yuan Wu;Xiudan Zheng;Yubo Ding;Min Zhou;Zhuang Wei;Tao Liu;Kan Liao
通讯作者：
Kan Liao
影响因子：
4
作者：
P. Csermely;T. Schnaider;Z. Prohászka;G. Nardai
通讯作者：
P. Csermely;T. Schnaider;Z. Prohászka;G. Nardai
通过 FT-IR 光谱评估曲古抑菌素 A 对 He La 细胞的影响
影响因子：
7.4
作者：
Zhang Fengqiu;Huang Qing;Yan Jingwen;Zhang Xin;Li Jianxin
通讯作者：
Li Jianxin
影响因子：
4.8
作者：
Giustiniani, Julien;Daire, Vanessa;Baillet, Anita
通讯作者：
Baillet, Anita
影响因子：
11.4
作者：
Bravo-Cordero, Jose J.;Marrero-Diaz, Raquel;Montoya, Maria C.
通讯作者：
Montoya, Maria C.