The anti-inflammatory activity of HMGB1 A box is enhanced when fused with C-terminal acidic tail.
The anti-inflammatory activity of HMGB1 A box is enhanced when fused with C-terminal acidic tail.
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DOI：
10.1155/2010/915234
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发表时间：
2010
影响因子：
--
通讯作者：
He F
中科院分区：
文献类型：
--
作者：
Gong W;Zheng Y;Chao F;Li Y;Xu Z;Huang G;Gao X;Li S;He F
HMGB1由A盒、B盒和C尾区组成，是一种重要的促炎细胞因子，参与多种炎症性疾病。B盒介导促炎活性，而A盒单独作为HMGB1的特异性拮抗剂。C尾对A盒的空间结构有贡献，并调节HMGB1 DNA结合的特异性。目前尚不清楚C尾是否能增强A盒的抗炎作用。在本研究中，我们构建了由A盒和C尾组成的融合蛋白，其中B盒被删除，A盒和C尾直接连接或通过柔性连接序列(Gly4Ser)3连接。体内外实验表明，这两种融合蛋白比单独的A盒具有更高的抗炎活性。这表明融合的C尾巴增强了A盒的抗炎作用。
HMGB1, composed of the A box, B box, and C tail domains, is a critical proinflammatory cytokine involved in diverse inflammatory diseases. The B box mediates proinflammatory activity, while the A box alone acts as a specific antagonist of HMGB1. The C tail contributes to the spatial structure of A box and regulates HMGB1 DNA binding specificity. It is unknown whether the C tail can enhance the anti-inflammatory effect of A box. In this study, we generated fusion proteins consisting of the A box and C tail, in which the B box was deleted and the A box and C tail were linked either directly or by the flexible linker sequence (Gly4Ser)3. In vitro and in vivo experiments showed that the two fusion proteins had a higher anti-inflammatory activity compared to the A box alone. This suggests that the fused C tail enhances the anti-inflammatory effect of the A box.
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DOI：
10.1097/shk.0b013e3181997173
发表时间：
2009-07
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Peltz ED;Moore EE;Eckels PC;Damle SS;Tsuruta Y;Johnson JL;Sauaia A;Silliman CC;Banerjee A;Abraham E
通讯作者：
Abraham E
影响因子：
64.8
作者：
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通讯作者：
Bianchi, ME
影响因子：
11.1
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Müller, S;Ronfani, L;Bianchi, ME
通讯作者：
Bianchi, ME
影响因子：
2.9
作者：
Knapp, S;Müller, S;Musco, G
通讯作者：
Musco, G
DOI：
10.1016/j.bbrc.2007.05.130
发表时间：
2007-08-17
影响因子：
3.1
作者：
Wang, Qiyu;Zeng, Minghui;Tang, Jie
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Tang, Jie