Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans.
Myotactin, a novel hypodermal protein involved in muscle-cell adhesion in Caenorhabditis elegans.
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DOI：
10.1083/jcb.146.3.659
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发表时间：
1999-08-09
期刊：
影响因子：
--
通讯作者：
Waterston RH
中科院分区：
文献类型：
--
作者：
Hresko MC;Schriefer LA;Shrimankar P;Waterston RH
In C.在elegans中，被称为纤维细胞器的下皮半桥粒样结构的组装与肌肉收缩装置的组装在时间和空间上协调，表明这些细胞类型之间交换信号以正确定位纤维细胞器。Myotactin是一种被单克隆抗体MH 46识别的蛋白质，是这种信号分子的候选者。抗原虽然由下皮表达，但首先反映肌肉成分的模式，随后才反映纤维细胞器的模式。共聚焦显微镜显示，在蠕虫肌动蛋白和纤维细胞器显示一致的定位。此外，细胞消融研究表明，体壁肌细胞是正常的肌肌动蛋白分布所必需的。为了研究肌动素在肌肉-皮下信号传导中的作用，我们从分子和遗传学上表征了肌动素位点。Myotactin是一种分子量约为500 kd的新型跨膜蛋白。胞外结构域含有至少32个纤连蛋白III型重复序列，胞质结构域含有独特序列。在缺乏肌动素的突变体中，当胚胎肌肉收缩开始时，肌肉细胞分离。在发育后期，纤维细胞器变得离域，并且不限于先前与肌肉接触的皮下组织区域。这些结果表明，myotactin有助于维持肌肉收缩装置和皮下纤维细胞器之间的联系。
In C. elegans, assembly of hypodermal hemidesmosome-like structures called fibrous organelles is temporally and spatially coordinated with the assembly of the muscle contractile apparatus, suggesting that signals are exchanged between these cell types to position fibrous organelles correctly. Myotactin, a protein recognized by monoclonal antibody MH46, is a candidate for such a signaling molecule. The antigen, although expressed by hypodermis, first reflects the pattern of muscle elements and only later reflects the pattern of fibrous organelles. Confocal microscopy shows that in adult worms myotactin and fibrous organelles show coincident localization. Further, cell ablation studies show the bodywall muscle cells are necessary for normal myotactin distribution. To investigate myotactin's role in muscle-hypodermal signaling, we characterized the myotactin locus molecularly and genetically. Myotactin is a novel transmembrane protein of ∼500 kd. The extracellular domain contains at least 32 fibronectin type III repeats and the cytoplasmic domain contains unique sequence. In mutants lacking myotactin, muscle cells detach when embryonic muscle contraction begins. Later in development, fibrous organelles become delocalized and are not restricted to regions of the hypodermis previously contacted by muscle. These results suggest myotactin helps maintain the association between the muscle contractile apparatus and hypodermal fibrous organelles.
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影响因子：
7.8
作者：
BARNIK, E;OSBORN, M;WEBER, K
通讯作者：
WEBER, K
DOI：
10.1083/jcb.101.4.1532
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Francis GR;Waterston RH
通讯作者：
Waterston RH
DOI：
10.1083/jcb.124.4.491
发表时间：
1994-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hresko MC;Williams BD;Waterston RH
通讯作者：
Waterston RH
影响因子：
7.8
作者：
BARSTEAD, RJ;WATERSTON, RH
通讯作者：
WATERSTON, RH
DOI：
10.1089/cmb.1995.2.9
发表时间：
1995-01-01
期刊：
Journal of computational biology : a journal of computational molecular cell biology
影响因子：
--
作者：
Eddy, S R;Mitchison, G;Durbin, R
通讯作者：
Durbin, R