CM2 antigen, a potential novel molecule participating in glucuronide transport on rat hepatocyte canalicular membrane.
CM2 antigen, a potential novel molecule participating in glucuronide transport on rat hepatocyte canalicular membrane.
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CM2抗原，一种参与大鼠肝细胞小管膜上葡萄糖苷酸转运的潜在新分子
DOI：
10.4081/ejh.2012.e26
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发表时间：
2012-06-29
期刊：
影响因子：
--
通讯作者：
Han Y
中科院分区：
文献类型：
--
作者：
Wang L;Wang J;Zhou X;Li J;Shi Y;Han Z;Wang X;Li S;Yang Z;Wang R;Fan D;Han Y
主要分布于肝细胞小管表面的极化分子在揭示胆汁形成过程和胆汁淤积性肝病的发病机制中起着重要作用。因此，寻找新的肝细胞小管膜极化分子具有重要意义。本研究以密度梯度离心法从大鼠肝细胞中分离的小管膜囊泡（CMVs）为免疫原，制备杂交瘤，获得46株抗CMVs的单克隆抗体（mAb）。通过免疫组化、免疫荧光和免疫电镜等一系列形态学检测方法，证实了微管单抗1（CM 1）和微管单抗2（CM 2）识别的抗原主要分布在肝细胞微管膜上。转运活性实验表明，CM2可抑制大鼠肝细胞CMV对E217βG的摄取。同时，Western blotting分析表明CM2抗原的分子量约为110 kDa，远小于参与葡糖苷酸转运的多药耐药相关蛋白2（MRP2）的分子量（180 kDa）。这些数据表明，CM2抗原可能是一个潜在的新分子参与葡萄糖醛酸苷转运的肝细胞小管膜。
The polarized molecules predominately distributing at hepatocyte canalicular surface play a vital role in disclosing the process of bile formation and etiopathogenisis of cholestatic live diseases. Therefore, it is important to find novel polarized molecules on hepatocyte canalicular membrane. In the present study, canalicular membrane vesicles (CMVs) isolated from rat hepatocyte by density gradient centrifugation were used as immunogens to produce hybridoma and 46 strains of monoclonal antibodies (mAb) against CMVs were obtained. With a series of morphological assay methods, including immunohistochemistry, immunofluorescence and immuno-electron microscope, the antigens recognized by canalicular mAb1 (CM1) and canalicular mAb2 (CM2) were confirmed to predominately distribute at hepatocyte canalicular membrane. Transport activity assay revealed that CM2 could inhibit ATP-dependent E217βG uptake of rat hepatocyte CMVs. Meanwhile, Western blotting analysis showed that the molecular mass of CM2 antigen was approximately 110kDa, which was much less than Mr 180kDa of multidrug resistance-associated protein 2 (MRP2) involved in glucuronide transport. These data indicated that CM2 antigen might be a potential novel molecule participating in glucuronide transport on the hepatocyte canalicular membrane.
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影响因子：
2.5
作者：
Dean, Michael
通讯作者：
Dean, Michael
影响因子：
2.9
作者：
TIETZ, PS;HOLMAN, RT;LARUSSO, NF
通讯作者：
LARUSSO, NF
DOI：
10.1083/jcb.96.6.1548
发表时间：
1983-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Roman LM;Hubbard AL
通讯作者：
Hubbard AL
影响因子：
3.7
作者：
Kouzuki, H;Suzuki, H;Sugiyama, Y
通讯作者：
Sugiyama, Y
影响因子：
46.9
作者：
Scherer, PE;Bickel, PE;Lodish, HF
通讯作者：
Lodish, HF