Mutagenic metabolites in urine and feces of rats fed with 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, a carcinogenic mutagen present in cooked meat.
Mutagenic metabolites in urine and feces of rats fed with 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, a carcinogenic mutagen present in cooked meat.
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喂食 2-氨基-3,8-二甲基咪唑[4,5-f]喹喔啉（一种存在于熟肉中的致癌诱变剂）的大鼠尿液和粪便中的致突变代谢物。
作者：
H. Hayatsu;H. Kasai;S. Yokoyama;T. Miyazawa;Z. Yamaizumi;S. Sato;S. Nishimura;S. Arimoto;T. Hayatsu;Y. Ohara
为了研究肉类致癌物2-氨基-3,8-二甲基咪唑[4,5-f]喹诺啉（MeIQx）在大鼠体内的作用，在大鼠的饮食中添加了MeIQx，并对其尿液和粪便进行了代谢分析。分离过程包括将MeIQx衍生物在蓝棉上进行特异性吸附，然后通过硅胶薄层色谱和高压液相色谱进行分离。注意力集中在具有诱变活性的代谢物上。从尿液中分离出3种代谢物，并通过1H核磁共振、紫外和质谱对其结构进行了鉴定。鉴定的第一个代谢物为2-氨基-8-羟甲基-3-甲基咪唑[4,5-f]喹啉（化合物I），第二个代谢物为2-乙酰氨基-3,8-二甲基咪唑[4,5-f]喹诺啉（化合物II），第三个代谢物为2-氨基-8-甲基咪唑[4,5-f]喹诺啉（化合物III）。化合物1也从粪便中分离得到。化合物I-III对鼠伤寒沙门菌TA98具有代谢激活诱变作用。化合物I和II的诱变效力与MeIQx相当，而化合物III的诱变效力远低于MeIQx。
To study the in vivo fate of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), a carcinogenic mutagen present in cooked meat, rats were fed MeIQx in the diet and their urine and feces were analyzed for the metabolites. The isolation procedure included specific adsorption of MeIQx derivatives to blue cotton and subsequent fractionations by thin layer chromatography on silica gel and by high pressure liquid chromatography. Attention was focused on mutagenically active metabolites. Three metabolites were isolated from the urine, and their structures were elucidated on the basis of 1H nuclear magnetic resonance, ultraviolet, and mass spectra. The first metabolite characterized was 2-amino-8-hydroxymethyl-3-methylimidazo[4,5-f]quinoxaline (Compound I), the second was 2-acetylamino-3,8-dimethylimidazo[4,5-f]quinoxaline (Compound II), and the third was 2-amino-8-methylimidazo[4,5-f]quinoxaline (Compound III). Compound I was isolated also from the feces. Compounds I-III were mutagenic to Salmonella typhimurium TA98 with metabolic activation. The mutagenic potency of Compounds I and II was as high as that of MeIQx, and that of Compound III was much lower than that of MeIQx.
商业牛肉提取物中诱变剂的定性和定量表征。
影响因子：
4.7
作者：
Turesky,RJ;Wishnok,JS;Tannenbaum,SR;Pfund,RA;Buchi,GH
通讯作者：
Buchi,GH