Going global: the new era of mapping modifications in RNA.
Going global: the new era of mapping modifications in RNA.
复制标题
DOI：
10.1002/wrna.1367
复制
发表时间：
2017-01
影响因子：
7.3
通讯作者：
Paulines, Mellie June
中科院分区：
文献类型：
--
作者：
Limbach, Patrick A.;Paulines, Mellie June
已知通过添加一个或多个化学基团对RNA进行转录后修饰已有50多年。这些化学修饰，曾经被认为是静态的，现在被发现在基因表达中起着关键的调节作用。大规模并行RNA测序（RNA-seq）的出现现在使我们能够探索细胞RNA的复杂性以及化学改变RNA结构如何扩展RNA词汇。在这里，我们提出了一个概述的各种策略和技术，可用于在细胞水平上的RNA化学修饰。这些策略可以被表征为靶向和非靶向的方法：靶向策略是针对一个单一的化学修饰而开发的，而非靶向策略更广泛地适用于一系列这样的化学变化。所有这些方法的关键是定位RNA序列内修饰的能力。虽然这些方法大多建立在RNA-Seq管道上，但基于质谱或常规DNA测序的替代方法在整个分析过程中保留了价值。我们还期待未来的机会和技术，可能会扩大可以在全球范围内分析的修改类型。鉴于越来越多的认识，这些RNA化学修饰发挥重要的生物学作用，各种方法，优选正交方法，将需要在全球范围内识别，验证和定量RNA的转录组中发现的化学修饰。
The post-transcriptional modification of RNA by the addition of one or more chemical groups has been known for over 50 years. These chemical modifications, once thought to be static, are now being discovered to play key regulatory roles in gene expression. The advent of massive parallel sequencing of RNA (RNA-seq) now allows us to probe the complexity of cellular RNA and how chemically altering RNA structure expands the RNA vocabulary. Here we present an overview of the various strategies and technologies that are available to profile RNA chemical modifications at the cellular level. These strategies can be characterized as targeted and untargeted approaches: targeted strategies are developed for one single chemical modification while untargeted strategies are more broadly applicable to a range of such chemical changes. Key for all of these approaches is the ability to locate modifications within the RNA sequence. While most of these methods are built upon an RNA-Seq pipeline, alternative approaches based on mass spectrometry or conventional DNA sequencing retain value in the overall analysis process. We also look forward toward future opportunities and technologies that may expand the types of modifications that can be globally profiled. Given the ever increasing recognition that these RNA chemical modifications play important biological roles, a variety of methods, preferably orthogonal approaches, will be required to globally identify, validate and quantify RNA chemical modifications found in the transcriptome.
登录
查看更多内容
影响因子：
14.9
作者：
Ebhardt HA;Tsang HH;Dai DC;Liu Y;Bostan B;Fahlman RP
通讯作者：
Fahlman RP
影响因子：
14.9
作者：
Hauenschild R;Tserovski L;Schmid K;Thüring K;Winz ML;Sharma S;Entian KD;Wacheul L;Lafontaine DL;Anderson J;Alfonzo J;Hildebrandt A;Jäschke A;Motorin Y;Helm M
通讯作者：
Helm M
影响因子：
14.9
作者：
Torres AG;Piñeyro D;Rodríguez-Escribà M;Camacho N;Reina O;Saint-Léger A;Filonava L;Batlle E;Ribas de Pouplana L
通讯作者：
Ribas de Pouplana L
影响因子：
8.8
作者：
Hussain S;Sajini AA;Blanco S;Dietmann S;Lombard P;Sugimoto Y;Paramor M;Gleeson JG;Odom DT;Ule J;Frye M
通讯作者：
Frye M
影响因子：
14.9
作者：
BAKIN, A;KOWALAK, JA;OFENGAND, J
通讯作者：
OFENGAND, J