Identification of the Hub Genes in Alzheimer's Disease.
Identification of the Hub Genes in Alzheimer's Disease.
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DOI：
10.1155/2021/6329041
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发表时间：
2021
影响因子：
--
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Gui H;Gong Q;Jiang J;Liu M;Li H
阿尔茨海默病（Alzheimer's disease，AD）是最常见的神经退行性疾病，也是影响老年人的主要致死性疾病之一，给社会带来了巨大的负担。因此，识别AD相关的枢纽基因对于开发抗AD的新策略是极其重要的。 在这里，我们从美国国家生物技术信息中心（NCBI）GEO数据库中提取基因表达谱GSE 63061。一旦未经验证的基因芯片被删除，我们标准化后的微阵列数据的质量控制。我们利用Limma软件包筛选差异表达基因（DEG）。我们进行了基因本体论（GO）和京都基因和基因组百科全书（KEGG）分析的DEG。随后，我们使用STRING数据库构建了一个蛋白质-蛋白质相互作用（PPI）网络。 我们筛选了2169个DEG，包括1313个上调的DEG和856个下调的DEG。功能富集分析显示，免疫应答、中性粒细胞脱颗粒、溶酶体、破骨细胞分化等功能在表达上调的DEGs中富集;肽生物合成、翻译、核糖体、氧化磷酸化等功能在表达下调的DEGs中富集。在上调DEG构建的PPI网络中显示了379个节点和1149条PPI边;在下调DEG构建的PPI网络中显示了202个节点和1963条PPI边。四个枢纽基因，包括GAPDH，RHOA，RPS 29和RPS 27 A，被鉴定为参与AD病理学的新产生的候选基因。 GAPDH、RHOA、RPS 29和RPS 27 A预计是AD进展的关键候选物。本研究的结果可以为了解AD的发病机制和潜在的新治疗靶点提供全面的见解。
Alzheimer's disease (AD) is considered to be the most common neurodegenerative disease and also one of the major fatal diseases affecting the elderly, thus bringing a huge burden to society. Therefore, identifying AD-related hub genes is extremely important for developing novel strategies against AD. Here, we extracted the gene expression profile GSE63061 from the National Center for Biotechnology Information (NCBI) GEO database. Once the unverified gene chip was removed, we standardized the microarray data after quality control. We utilized the Limma software package to screen the differentially expressed genes (DEGs). We conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs. Subsequently, we constructed a protein-protein interaction (PPI) network using the STRING database. We screened 2169 DEGs, comprising 1313 DEGs with upregulation and 856 DEGs with downregulation. Functional enrichment analysis showed that the response of immune, the degranulation of neutrophils, lysosome, and the differentiation of osteoclast were greatly enriched in DEGs with upregulation; peptide biosynthetic process, translation, ribosome, and oxidative phosphorylation were dramatically enriched in DEGs with downregulation. 379 nodes and 1149 PPI edges were demonstrated in the PPI network constructed by upregulated DEGs; 202 nodes and 1963 PPI edges were shown in the PPI network constructed by downregulated DEGs. Four hub genes, including GAPDH, RHOA, RPS29, and RPS27A, were identified to be the newly produced candidates involved in AD pathology. GAPDH, RHOA, RPS29, and RPS27A are expected to be key candidates for AD progression. The results of this study can provide comprehensive insight into understanding AD's pathogenesis and potential new therapeutic targets.
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