Autophagy attenuates particulate matter 2.5-induced damage in HaCaT cells.
Autophagy attenuates particulate matter 2.5-induced damage in HaCaT cells.
复制标题
自噬减弱HaCaT细胞中颗粒物质2.5诱导的损伤。
DOI：
10.21037/atm-21-2146
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发表时间：
2021-06
影响因子：
--
通讯作者：
Peng X
中科院分区：
文献类型：
--
作者：
Dai Y;Wang Y;Lu S;Deng X;Niu X;Guo Z;Qian R;Zhou M;Peng X
角质形成细胞是皮肤屏障的关键组成部分，维持皮肤稳态。 PM2.5作为一种环境致病因子，可引起表皮细胞损伤，但其机制仍有待阐明。本研究旨在评估 PM2.5 对 HaCaT 细胞的影响并探讨其潜在机制。 HaCaT 细胞用 PM2.5 单独处理或与 UVB 照射结合处理 12 小时或 24 小时。采用细胞计数试剂盒 (CCK-8) 检测 PM2.5 对 HaCaT 细胞活力的影响。采用流式细胞术、Western Blot、AO染色检测细胞凋亡和自噬的变化。采用CCK-8和流式细胞术分析自噬抑制剂3-MA预处理后HaCaT细胞细胞毒性和凋亡的变化。结果表明，PM2.5通过增加细胞凋亡和激活自噬来诱导细胞毒性。自噬抑制后确定细胞凋亡显着增加。此外，太阳辐射加剧了 PM2.5 引起的 HaCaT 细胞损伤，从而进一步增强了自噬。但联合治疗抑制自噬后细胞凋亡无显着差异。我们的数据表明，PM2.5 会引起 HaCaT 细胞损伤，而自噬对促进细胞存活发挥保护作用。
Keratinocyte is a key component of the skin barrier and maintains skin homeostasis. As an environmental pathogenic factor, PM2.5 can cause epidermal cell damage, but the mechanism remains to be elucidated. The present study aimed to evaluate the effect caused by PM2.5 in HaCaT cells and investigate the underlying mechanisms. HaCaT cells were treated with PM2.5 for 12 h or 24 h, either alone or combined with UVB irradiation. A Cell Counting Kit (CCK-8) assay was carried out to detect the effect of PM2.5 on HaCaT cell viability. Flow cytometry, Western Blot, and AO staining were employed to detect the changes of apoptosis and autophagy. The changes of cytotoxicity and apoptosis in HaCaT cells were analyzed by CCK-8 and flow cytometry after pretreatment with autophagy inhibitor 3-MA. The results showed that PM2.5 induced cytotoxicity by increasing cell apoptosis and activating autophagy. Apoptosis was determined to be increased significantly after autophagy inhibition. Moreover, solar radiation intensified PM2.5-induced damage in HaCaT cells, which further enhanced the autophagy. However, there was no significant difference in apoptosis after inhibition of autophagy in combined treatment. Our data reveals that PM2.5 induces damage in HaCaT cells, and autophagy plays a protective role to promote cell survival.
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影响因子：
10.4
作者：
Kioumourtzoglou MA;Schwartz JD;Weisskopf MG;Melly SJ;Wang Y;Dominici F;Zanobetti A
通讯作者：
Zanobetti A
影响因子：
8
作者：
Kim, Byung Eui;Kim, Jihyun;Ahn, Kangmo
通讯作者：
Ahn, Kangmo
影响因子：
8.3
作者：
Oh, Inbo;Lee, Jiho;Kim, Yangho
通讯作者：
Kim, Yangho
影响因子：
6.8
作者：
Qiu, Yi-Ning;Wang, Guo-Hui;Zhang, Ke-Zhong
通讯作者：
Zhang, Ke-Zhong
影响因子：
7.7
作者：
Hayes, Richard B.;Lim, Chris;Thurston, George D.
通讯作者：
Thurston, George D.