Clearance of maternal barriers by paternal miR159 to initiate endosperm nuclear division in Arabidopsis.
Clearance of maternal barriers by paternal miR159 to initiate endosperm nuclear division in Arabidopsis.
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通过父本 miR159 清除母本屏障以启动拟南芥胚乳核分裂。
DOI：
10.1038/s41467-018-07429-x
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发表时间：
2018-11-27
影响因子：
16.6
通讯作者：
Zheng B
中科院分区：
文献类型：
--
作者：
Zhao Y;Wang S;Wu W;Li L;Jiang T;Zheng B
在种子发育过程中，精子进入触发了中央细胞分裂，但是除了基因组之外，还有哪些因素从精子中遗传，以及父系因素调节早期分裂事件的机制尚不清楚。在这里，我们表明，精子传输的miR159通过抑制中央细胞传输的miR159靶点来促进胚乳核分裂。父本miR159的破坏导致大约一半的种子由于胚乳核分裂缺陷而流产。在野生型植物中，MYB33和MYB65，两个miR159靶标，在受精前在中央细胞中高度表达，但在受精后两者都迅速消失。相反，父本miR159的缺失导致MYB33和MYB65在受精后保留在中央细胞中。此外，胚乳中miR159抗性形式的MYB33（mMYB33）的异位表达显著抑制胚乳核分裂的起始。总的来说，这些结果表明父本miR159抑制其母本靶点以促进胚乳核分裂，从而揭示了先前未知的父本对种子发育的影响。植物种子的发育是由精子进入胚珠引发的。在这里，Zhao等人发现miR159是种子发育的父源触发因子，它被传递到中央细胞，在中央细胞中抑制母体靶点的表达，以促进胚乳中的核分裂。
Sperm entry triggers central cell division during seed development, but what factors besides the genome are inherited from sperm, and the mechanism by which paternal factors regulate early division events, are not understood. Here we show that sperm-transmitted miR159 promotes endosperm nuclear division by repressing central cell-transmitted miR159 targets. Disruption of paternal miR159 causes approximately half of the seeds to abort as a result of defective endosperm nuclear divisions. In wild-type plants, MYB33 and MYB65, two miR159 targets, are highly expressed in the central cell before fertilization, but both are rapidly abolished after fertilization. In contrast, loss of paternal miR159 leads to retention of MYB33 and MYB65 in the central cell after fertilization. Furthermore, ectopic expression of a miR159-resistant version of MYB33 (mMYB33) in the endosperm significantly inhibits initiation of endosperm nuclear division. Collectively, these results show that paternal miR159 inhibits its maternal targets to promote endosperm nuclear division, thus uncovering a previously unknown paternal effect on seed development. Seed development in plants is triggered by entry of sperm to the ovule. Here, Zhao et al. uncover miR159 as a paternal-trigger of seed development that is transmitted to the central cell where it represses expression of maternal targets to promote nuclear division in the endosperm.
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影响因子：
4.3
作者：
Curtis MJ;Belcram K;Bollmann SR;Tominey CM;Hoffman PD;Mercier R;Hays JB
通讯作者：
Hays JB
影响因子：
11.6
作者：
Millar, AA;Gubler, F
通讯作者：
Gubler, F
影响因子：
7.4
作者：
Alonso-Peral, Maria M.;Li, Junyan;Millar, Anthony A.
通讯作者：
Millar, Anthony A.
DOI：
10.1073/pnas.93.11.5319
发表时间：
1996-05-28
影响因子：
11.1
作者：
Ohad, N;Margossian, L;Fischer, RL
通讯作者：
Fischer, RL
影响因子：
2.7
作者：
Armenta-Medina, Alma;Lepe-Soltero, Daniel;Gillmor, C. Stewart
通讯作者：
Gillmor, C. Stewart