Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size
Multi-stage premix membrane emulsification for preparation of agarose microbeads with uniform size
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多级预混膜乳化制备大小均一的琼脂糖微珠
DOI：
10.1016/j.memsci.2008.05.025
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发表时间：
2008-09
影响因子：
9.5
通讯作者：
Su Zhiguo
中科院分区：
文献类型：
--
作者：
Wang Lianyan;Zhou Qingzhu;Ma Guanghui;Su Zhiguo
采用预混合膜乳化法制备了粒径小于10μm、琼脂糖含量高达14wt%的琼脂糖珠。采用琼脂糖水溶液作为水相。以含六甘五酯（PO-500）的液体石蜡和石油醚的混合物为油相。将水相与油相在60℃下混合，在均质机中制备粗W/O乳液。然后，将粗乳液在高压下通过疏水膜挤压形成乳液，在温和搅拌下缓慢冷却形成凝胶珠。研究了制备条件对乳化效果的影响，结果表明：压力、道次、油相中石油醚/液体石蜡（v/v）、PO-500浓度和琼脂糖浓度对乳化效果的影响最大；粗乳不影响乳化效果。在最佳制备条件下，琼脂糖珠的变异系数为9.8%。该方法实现了常规乳液法难以制备的尺寸均匀且琼脂糖含量高的微珠。
Uniform-sized agarose beads with diameters less than 10μm and agarose content as high as 14wt% were prepared by premix membrane emulsification. Agarose aqueous solution was used as the water phase. A mixture of liquid paraffin and petroleum ether containing hexaglycerin penta ester (PO-500) was used as the oil phase. The water phase was mixed with the oil phase at 60°C and a coarse W/O emulsion was produced in a homogenizer. Then, the coarse emulsion was extruded through a hydrophobic membrane under high pressure to form an emulsion, which was slowly cooled under gentle agitation to form gel beads. The effects of preparation conditions on emulsification results were investigated and it showed that the pressure, number of passes, petroleum ether/liquid paraffin (v/v) in the oil phase, the concentration of PO-500 and concentration of agarose in the water phase, all affected the size and uniformity; coarse emulsion did not affect the emulsification results. The coefficient variation (C.V.) of agarose beads under optimal preparation conditions was 9.8%. This method realized microbeads with both uniform sizes and high agarose contents that are difficult to be prepared by conventional emulsion methods.
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影响因子：
4.1
作者：
H. Pertoft;A. Hallén
通讯作者：
H. Pertoft;A. Hallén
DOI：
10.1007/978-94-009-5938-5_1
发表时间：
1977
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
R. Hamilton;P. Sewell
通讯作者：
R. Hamilton;P. Sewell
DOI：
10.1016/s0021-9673(00)96091-6
发表时间：
1990-02
期刊：
Journal of chromatography
影响因子：
--
作者：
S. Hjertén;J. Li
通讯作者：
S. Hjertén;J. Li
影响因子：
9.5
作者：
G. Vladisavljević;M. Shimizu;T. Nakashima
通讯作者：
G. Vladisavljević;M. Shimizu;T. Nakashima
DOI：
10.1016/j.colsurfa.2003.08.005
发表时间：
2003-11-24
影响因子：
5.2
作者：
Kobayashi, I;Nakajima, M;Mukataka, S
通讯作者：
Mukataka, S