Electroacupuncture decreases excessive alcohol consumption involving reduction of FosB/ΔFosB levels in reward-related brain regions.
Electroacupuncture decreases excessive alcohol consumption involving reduction of FosB/ΔFosB levels in reward-related brain regions.
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DOI：
10.1371/journal.pone.0040347
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ye JH
中科院分区：
文献类型：
--
作者：
Li J;Sun Y;Ye JH
酒精滥用是美国和世界范围内的一个主要公共卫生问题，需要新的治疗方法。只有三种FDA批准的药物用于治疗酒精滥用（纳洛酮，阿坎酸和双磺草胺）。平均而言，这些药物在减少长期饮酒方面仅取得中等成功。电针已经被证明可以减轻各种药物的滥用，包括酒精。虽然以前的研究表明，电针可以减少饮酒，但其潜在机制尚未完全阐明。ΔFosB和FosB是与药物成瘾的神经可塑性有关的转录因子Fos家族的成员;电针治疗酒精滥用和Fos家族之间的联系尚未建立。在这项研究中，我们训练大鼠饮用大量的乙醇在一个修改的间歇性访问两瓶选择饮用程序。当大鼠达到一个稳定的基线乙醇消耗量，电针（100 Hz或2 Hz，30 min/d）足三里（ST 36）连续6天。免疫组化检测奖励相关脑区FosB/ΔFosB的表达。结果发现，100 Hz电针组大鼠对乙醇的摄入量和偏好性明显降低，而2 Hz电针组无明显变化。电针治疗结束后，这种减少至少维持72小时。相反，100赫兹电针并没有改变天然奖励剂蔗糖的摄入量和偏好。此外，过量饮酒后，前额叶皮层、纹状体区和腹侧被盖区后部的FosB/ΔFosB水平升高，而100 Hz电针6 d后，FosB/Δ FosB水平降低。因此，这项研究表明，为期六天的100 Hz电针治疗有效地减少了长期饮用过量乙醇的大鼠的乙醇消耗和偏好。电针的这种效应可能是通过下调奖赏相关脑区FosB/ΔFosB的表达来介导的。
New therapies are needed for alcohol abuse, a major public health problem in the U.S. and worldwide. There are only three FDA-approved drugs for treatment of alcohol abuse (naltrexone, acamprosate and disulfuram). On average these drugs yield only moderate success in reducing long-term alcohol consumption. Electroacupuncture has been shown to alleviate various drugs of abuse, including alcohol. Although previous studies have shown that electroacupuncture reduced alcohol consumption, the underlying mechanisms have not been fully elucidated. ΔFosB and FosB are members of the Fos family of transcription factors implicated in neural plasticity in drug addiction; a connection between electroacupuncture's treatment of alcohol abuse and the Fos family has not been established. In this study, we trained rats to drink large quantities of ethanol in a modified intermittent access two-bottle choice drinking procedure. When rats achieved a stable baseline of ethanol consumption, electroacupuncture (100 Hz or 2 Hz, 30 min each day) was administered at Zusanli (ST36) for 6 consecutive days. The level of FosB/ΔFosB in reward-related brain regions was assessed by immunohistochemistry. We found that the intake of and preference for ethanol in rats under 100 Hz, but not 2 Hz electroacupuncture regiment were sharply reduced. The reduction was maintained for at least 72 hours after the termination of electroacupuncture treatment. Conversely, 100 Hz electroacupuncture did not alter the intake of and preference for the natural rewarding agent sucrose. Additionally, FosB/ΔFosB levels in the prefrontal cortex, striatal region and the posterior region of ventral tegmental area were increased following excessive ethanol consumption, but were reduced after six-day 100 Hz electroacupuncture. Thus, this study demonstrates that six-day 100 Hz electroacupuncture treatment effectively reduces ethanol consumption and preference in rats that chronically drink excessive amount of ethanol. This effect of electroacupuncture may be mediated by down-regulation of FosB/ΔFosB in reward-related brain regions.
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DOI：
10.1523/jneurosci.2243-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Jeanblanc J;He DY;Carnicella S;Kharazia V;Janak PH;Ron D
通讯作者：
Ron D
影响因子：
6.1
作者：
Marinelli, PW;Kiianmaa, K;Gianoulakis, C
通讯作者：
Gianoulakis, C
影响因子：
--
作者：
LU, GW
通讯作者：
LU, GW
影响因子：
8.1
作者：
Han, Jisheng;Cui, Cailian;Wu, Liuzhen
通讯作者：
Wu, Liuzhen
DOI：
10.1097/00000374-199908000-00002
发表时间：
1999-08-01
影响因子：
3.2
作者：
Fadda, P;Tronci, S;Fratta, W
通讯作者：
Fratta, W