Steady-state of an enzymatic reaction is dependent on the density of reactant.
Steady-state of an enzymatic reaction is dependent on the density of reactant.
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DOI：
10.1021/la3034066
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发表时间：
2013-01-08
期刊：
影响因子：
--
通讯作者：
Mrksich M
中科院分区：
文献类型：
--
作者：
Li S;Liao X;Mrksich M
蛋白质的翻译后修饰是由两种相对酶的相对活性控制的。例如，酪氨酸残基的磷酸化程度反映了激酶和磷酸酶的平衡。本文使用作为一个模型系统的自组装单层，提出了一种肽，可以通过Abl激酶磷酸化，随后通过λ磷酸酶去磷酸化。用含有两种酶的反应混合物处理单层揭示了肽磷酸化的稳态水平取决于肽的密度。使用相同的反应混合物，以高密度呈现底物的表面导致稳态的磷酸化肽，而以低密度呈现底物的表面导致稳态的非磷酸化肽。这种依赖性归因于在高密度底物下进行的自催化磷酸化反应。这项工作提供了一个界面反应的一个例子，具有在相应的溶液相反应中没有类似的性质。它还提供了一个模型系统，是相关的理解机制，调节信号在细胞膜。
The post-translational modification of proteins is controlled by the relative activities of two opposing enzymes. For example, the extent of phosphorylation of tyrosine residues reflects the balance of a kinase and a phosphatase enzyme. The present article uses as a model system a self-assembled monolayer that presents a peptide that can be phosphorylated by Abl kinase and subsequently dephosphorylated by Lambda phosphatase. Treatment of monolayers with a reaction mixture containing both enzymes reveals that the steady-state level of peptide phosphorylation is dependent on the density of the peptide. Using identical reaction mixtures, surfaces that presented the substrate at high density led to a phosphorylated peptide at steady-state, whereas surfaces that presented the substrate at low density led to unphosphorylated peptide at steady-state. This dependence owes to an autocatalytic phosphorylation reaction that operates at high densities of substrate. This work provides an example of an interfacial reaction that has properties that have no analogue in the corresponding solution phase reaction. It also provides a model system that is relevant to understanding mechanisms that regulate signaling at the cellular membrane.
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
3.9
作者：
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作者：
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DOI：
10.1073/pnas.96.21.11782
发表时间：
1999-10-12
影响因子：
11.1
作者：
Horan, N;Yan, L;Kahne, D
通讯作者：
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