Algicidal metabolites produced by Bacillus sp. strain B1 against Phaeocystis globosa
Algicidal metabolites produced by Bacillus sp. strain B1 against Phaeocystis globosa
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芽孢杆菌产生的杀藻代谢物。
DOI：
10.1007/s10295-013-1393-0
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
赵玲
中科院分区：
文献类型：
--
作者：
赵玲
近年来球形棕囊藻水华在我国沿海地区频繁爆发，造成了巨大的经济损失。本研究表明，芽孢杆菌属菌株B1，这是以前由我们的小组，是有效的调节球形假单胞菌分泌活性代谢产物。测定了菌株B1所产化合物的热稳定性、pH稳定性和分子量范围，结果表明这些化合物的杀藻活性不受pH和温度的影响。甲醇提取的溶藻化合物经ODS-A柱层析和HPLC分离纯化。通过四极杆飞行时间质谱法（Q-TOF-MS）进一步分析从HPLC中洗脱的对应于峰2-5的杀藻化合物。PeakView™软件基于准确的质量信息、同位素模式和MS-MS光谱确定对应于峰2-5的化合物为l-组氨酸、o-酪氨酸、N-乙酰组胺和尿刊酸。这些化合物对中肋骨条藻、东海原甲藻和赤潮异弯藻也有一定的杀灭作用。这是首次报道仅通过Q-TOF-MS和PeakView™软件鉴定细菌来源的杀藻化合物，这些化合物将来可能用作杀藻剂的成分。
The bloom of Phaeocystis globosa has broken out frequently in the coastal areas of China in recent years, which has led to substantial economic losses. This study shows that Bacillus sp. strain B1, which was previously identified by our group, is effective in regulating P. globosa by excreting active metabolites. Heat stability, pH stability and molecular weight range of the algicidal compounds from strain B1 were measured and the results demonstrated that the algicidal activities of these compounds were not affected by pH or temperature variation. The algicidal compounds extracted with methanol were isolated and purified by ODS-A column chromatography and HPLC. The algicidal compounds corresponding to peaks 2–5 eluted from HPLC were further analysed by quadrupole time-of-flight mass spectrometry (Q-TOF–MS). PeakView™ Software determined the compounds corresponding to peaks 2–5 to be l-histidine, o-tyrosine, N-acetylhistamine and urocanic acid on the basis of the accurate mass information, the isotopic pattern and MS–MS spectra. Furthermore, these compounds were also able to eliminate Skeletonema costatum, Prorocentrum donghaiense and Heterosigma akashiwo. This is the first report of bacteria-derived algicidal compounds being identified only by Q-TOF–MS and PeakView™ Software, and these compounds may be used as the constituents of algicides in the future.
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DOI：
--
发表时间：
1973-12
期刊：
--
影响因子：
--
作者：
J. Stein
通讯作者：
J. Stein
影响因子：
8.8
作者：
D. Zare;Kharidah Muhammad;M. Bejo;H. Ghazali
通讯作者：
D. Zare;Kharidah Muhammad;M. Bejo;H. Ghazali
影响因子：
2.2
作者：
Amaro, AM;Fuentes, MS;Suárez-Isla, BA
通讯作者：
Suárez-Isla, BA
影响因子：
4.4
作者：
K. Yoshikawa;K. Adachi;M. Nishijima;T. Takadera;Seiji Tamaki;K. Harada;K. Mochida;H. Sano
通讯作者：
K. Yoshikawa;K. Adachi;M. Nishijima;T. Takadera;Seiji Tamaki;K. Harada;K. Mochida;H. Sano
影响因子：
1.6
作者：
Cho, Ji Young
通讯作者：
Cho, Ji Young