Measurement of glycerol turnover by infusion of nonisotopic glycerol in normal and injured subjects.

Measurement of glycerol turnover by infusion of nonisotopic glycerol in normal and injured subjects.
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通过在正常和受伤受试者中输注非同位素甘油来测量甘油周转率。

DOI:
10.1152/ajpendo.1984.247.3.e405
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发表时间:
1984
期刊:
The American journal of physiology
影响因子:
--
通讯作者:
Kinney,JM
Kinney,JM
中科院分区:
--
文献类型:
--
作者:
Carpentier,YA;Jeevanandam,M;Robin,AP;Nordenstrom,J;Burr,RE;Leibel,RL;Hirsch,J;Elwyn,DH;Kinney,JM

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一种新的技术测量甘油周转在人类受试者提出了使用阶段输液未标记的甘油。在正常受试者和术后或急性病人中共进行了105次测量。在21名正常受试者的80次分期输液测量中,甘油周转率(Rt)与浓度(C) (Rt = 31 + 2.07C)之间的关系与其他同位素稀释的观察结果没有显著差异。在11例患者中,还通过稀释同位素甘油来测量甘油周转率。两种方法的平均值无显著差异。无论符号如何,个体值的平均差异为17%。这种一致倾向于验证两种方法的假设。分阶段输注方法的优点是,它是更快,更容易执行,并没有风险,由于管理放射性物质。本研究从三个阶段的过程中获得的证据表明,以250 μmol/min和600 μmol/min的连续速度连续注射两阶段,每次注射70分钟,在每个阶段开始时给予2mmol的初始剂量,可以获得令人满意的信息。同位素稀释;甘油间隙;甘油浓度;脂类分解;1983年12月27日提交1984年4月18日接受
A new technique for measuring glycerol turnover in human subjects is presented using a staged infusion of unlabeled glycerol. A total of 105 measurements was made in normal subjects and postoperative or acutely ill patients. The relation between glycerol turnover rate (Rt) and concentration (C) (Rt = 31 + 2.07C) from 80 staged infusion measurements in 21 normal subjects did not differ significantly from observations of others made by isotope dilution. In 11 patients, glycerol turnover was also measured by dilution of isotopic glycerol. Mean values did not differ significantly between the two methods. The average difference, regardless of sign, for individual values was 17%. This agreement tends to validate the assumptions of both methods. Advantages of the staged infusion method are that it is quicker and easier to perform and does not entail a risk due to administration of radioactive materials. Evidence obtained in this study from a three-stage procedure indicates that satisfactory information may be obtained from a two-stage infusion at successive rates of 250 and 600 μmol/min for 70 min each, with a prime of 2 mmol given as a bolus at the start of each stage.isotope dilution; glycerol clearance; glycerol concentration; lipolysis; futile cycleSubmitted on December 27, 1983Accepted on April 18, 1984
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