Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.
Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.
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胚状体的单细胞分析揭示了早期人类发育的谱系多样化路线图。
DOI：
10.1016/j.stem.2022.08.009
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发表时间：
2022-09-01
期刊：
影响因子：
23.9
通讯作者：
Fu, Jianping
中科院分区：
文献类型：
--
作者：
Zheng, Yi;Yan, Robin Zhexuan;Sun, Shiyu;Kobayashi, Mutsumi;Xiang, Lifeng;Yang, Ran;Goedel, Alexander;Kang, Yu;Xue, Xufeng;Esfahani, Sajedeh Nasr;Liu, Yue;Irizarry, Agnes M. Resto;Wu, Weisheng;Li, Yunxiu;Ji, Weizhi;Niu, Yuyu;Chien, Kenneth R.;Li, Tianqing;Shioda, Toshihiro;Fu, Jianping
Despite its clinical and fundamental importance, our understanding of early human development remains limited. Stem cell-derived, embryo-like structures (or embryoids) allowing studies of early development without using natural embryos can potentially help fill the knowledge gap of human development. Herein, transcriptome at the single-cell level of a human embryoid model was profiled at different time points. Molecular maps of lineage diversifications from the pluripotent human epiblast towards the amniotic ectoderm, primitive streak / mesoderm, and primordial germ cells were constructed and compared with in vivo primate data. The comparative transcriptome analyses reveal a critical role of NODAL signaling in human mesoderm and primordial germ cell specification, which is further functionally validated. Through comparative transcriptome analyses and validations with human blastocysts and in vitro cultured cynomolgus embryos, we further proposed stringent criteria for distinguishing between human blastocyst trophectoderm and early amniotic ectoderm cells. Fu and colleagues profiled a stem cell-derived microfluidic amniotic sac embryoid (μPASE) using single-cell RNA Sequencing. Molecular maps of lineage diversifications from the pluripotent human epiblast towards the amniotic ectoderm, primitive streak / mesoderm, and primordial germ cells were constructed and compared with in vivo primate data.
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影响因子：
41.2
作者：
Fu J;Warmflash A;Lutolf MP
通讯作者：
Lutolf MP
影响因子：
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作者：
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Warmflash, Aryeh
DOI：
10.1073/pnas.0401654101
发表时间：
2004-05-18
影响因子：
11.1
作者：
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Beck, F
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Buettner, Florian