Mechanical Strain Determines Cilia Length, Motility, and Planar Position in the Left-Right Organizer.
Mechanical Strain Determines Cilia Length, Motility, and Planar Position in the Left-Right Organizer.
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DOI：
10.1016/j.devcel.2018.04.007
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发表时间：
2018-05-07
影响因子：
11.8
通讯作者：
Kintner C
中科院分区：
文献类型：
--
作者：
Chien YH;Srinivasan S;Keller R;Kintner C
非洲爪哇左-右组织器(LRO)通过产生和感知定向纤毛流作为图案线索，打破了早期胚胎左右轴的对称性。为了执行这一过程，LRO含有不同类型的纤毛细胞，纤毛长度不同，无论它们是运动性的还是感觉性的，以及它们如何沿前后(A-P)平面轴定位纤毛。在这里，我们表明，这些不同的纤毛特征是在原肠形成期间的预期LRO中指定的，基于沿A-P轴定向的各向异性机械应变，并沿着内侧轴水平分级。该菌株通过作用于囊胚期存在的中胚层前模式(包括foxj1)来指导纤毛细胞的分化。我们认为，差异应变是一种分级的发育线索，在非洲爪蛙LRO的形成过程中，A-P平面轴的建立与纤毛长度、运动性和平面位置有关。在非洲爪哇胚胎中，左右体轴的建立是通过在左右组织器中产生和感觉到向左的流体流动来实现的。Chien等人的研究。表明，产生这种流动所需的纤毛分化模式是由原肠形成过程中分级和定向的机械应变决定的。
The Xenopus left-right organizer (LRO) breaks symmetry along the left-right axis of the early embryo by producing and sensing directed ciliary flow as a patterning cue. To carry out this process, the LRO contains different ciliated cell types that vary in cilia length, whether they are motile or sensory, and how they position their cilia along the anterior-posterior (A-P) planar axis. Here we show that these different cilia features are specified in the prospective LRO during gastrulation, based on anisotropic mechanical strain that is oriented along the A-P axis, and graded in levels along the medial-lateral axis. Strain instructs ciliated cell differentiation by acting on a mesodermal prepattern present at blastula stages, involving foxj1. We propose that differential strain is a graded, developmental cue, linking the establishment of an A-P planar axis to cilia length, motility, and planar location, during formation of the Xenopus LRO. The left-right body axis is established in Xenopus embryos by leftward fluid flow produced and sensed in the left-right organizer. Chien et al. show that the pattern of cilia differentiation required to produce this flow is determined by graded and oriented mechanical strain during gastrulation.
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影响因子：
16.6
作者：
Gomez JM;Chumakova L;Bulgakova NA;Brown NH
通讯作者：
Brown NH
影响因子：
4.6
作者：
Chu CW;Ossipova O;Ioannou A;Sokol SY
通讯作者：
Sokol SY
影响因子：
4.6
作者：
Campbell, Evan P.;Quigley, Ian K.;Kintner, Chris
通讯作者：
Kintner, Chris
影响因子：
4.6
作者：
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通讯作者：
Kintner, Chris
DOI：
10.1073/pnas.1005998107
发表时间：
2010-10-26
影响因子：
11.1
作者：
Hellman, Nathan E.;Liu, Yan;Drummond, Iain A.
通讯作者：
Drummond, Iain A.