Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments.
Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments.
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DOI：
10.1371/journal.pone.0098482
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Steck TL
中科院分区：
文献类型：
--
作者：
Lange Y;Ye J;Steck TL
已经表明，适度增加质膜胆固醇超过其生理设定点大大增加了内质网和线粒体池，从而引发多种反馈反应，使细胞胆固醇返回到其静息状态。问题是，这种稳态机制是否反映了细胞表面胆固醇靶向特定的细胞内位点或其在细胞器之间的一般平衡。我们现在表明，人成纤维细胞胆固醇可以从2-羟丙基-β-环糊精增加多达两倍，而不改变细胞表面池的大小。相反，基本上所有添加的胆固醇都迅速分散在细胞质膜中，使其总胆固醇含量增加了五倍。我们的结论是，质膜胆固醇的水平通常是在容量，即使是小增量以上的生理设定点重新分配基本上完全细胞内膜，也许他们的化学活性梯度。
It has been shown that modestly increasing plasma membrane cholesterol beyond its physiological set point greatly increases the endoplasmic reticulum and mitochondrial pools, thereby eliciting manifold feedback responses that return cell cholesterol to its resting state. The question arises whether this homeostatic mechanism reflects the targeting of cell surface cholesterol to specific intracellular sites or its general equilibration among the organelles. We now show that human fibroblast cholesterol can be increased as much as two-fold from 2-hydroxypropyl-β-cyclodextrin without changing the size of the cell surface pool. Rather, essentially all of the added cholesterol disperses rapidly among cytoplasmic membranes, increasing their overall cholesterol content by as much as five-fold. We conclude that the level of plasma membrane cholesterol is normally at capacity and that even small increments above this physiological set point redistribute essentially entirely to intracellular membranes, perhaps down their chemical activity gradients.
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影响因子：
4.8
作者：
Lange, Y;Ye, J;Steck, TL
通讯作者：
Steck, TL
影响因子：
21.3
作者：
Feng, B;Yao, PM;Tabas, I
通讯作者：
Tabas, I
DOI：
10.1111/j.1600-0854.2011.01234.x
发表时间：
2011-10
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Georgiev AG;Sullivan DP;Kersting MC;Dittman JS;Beh CT;Menon AK
通讯作者：
Menon AK
影响因子：
7.5
作者：
Maxfield FR;van Meer G
通讯作者：
van Meer G
影响因子：
2.9
作者：
Lange, Yvonne;Ye, Jin;Steck, Theodore L.
通讯作者：
Steck, Theodore L.