Substance P-expressing Neurons in the Superficial Dorsal Horn of the Mouse Spinal Cord: Insights into Their Functions and their Roles in Synaptic Circuits.
Substance P-expressing Neurons in the Superficial Dorsal Horn of the Mouse Spinal Cord: Insights into Their Functions and their Roles in Synaptic Circuits.
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DOI：
10.1016/j.neuroscience.2020.06.038
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发表时间：
2020-12-01
期刊：
影响因子：
3.3
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Polgár E;Bell AM;Gutierrez-Mecinas M;Dickie AC;Akar O;Costreie M;Watanabe M;Todd AJ
物质P-表达放射状细胞在第二层收到一半的兴奋性突触输入从其他中间神经元。它们优先受GRP-eGFP小鼠系中表达eGFP的瞬时中枢细胞的神经支配。大约40%的投射神经元在第一层表达Tac 1，基因的物质P。沉默Tac 1细胞在背角减少反射反应冷和辐射热。速激肽P物质（SP）在脊髓背角浅层的许多中间神经元和一些投射神经元上均有表达。我们最近发现，SP-表达兴奋性中间神经元在第二层主要对应于一个形态类称为放射状细胞。然而，人们对它们的功能或它们的突触连接知之甚少。在这里，我们使用修改的Brainbow技术来定义兴奋性突触输入SP放射状细胞。我们发现，大约一半的兴奋性突触（由荷马的表达确定）是从与VGLUT 2，这可能主要来自本地interneurons的扣。其余的突触可能包括初级传入神经，它们通常具有非常低的VGLUT 2水平。我们的研究结果还表明，SP细胞的优先支配的兴奋性interneurons定义的绿色荧光蛋白的表达下的基因控制的胃泌素释放肽的人口，他们收到稀疏的输入从其他类型的兴奋性interneurons。我们发现，约40%的第一层投射神经元表达Tac 1，编码P物质的基因。最后，我们发现，沉默Tac 1表达细胞在背角的结果在反射反应显着减少冷和辐射热，但不影响撤回冯弗雷头发，或氯喹诱发的瘙痒。
Substance P-expressing radial cells in lamina II receive half of their excitatory synaptic input from other interneurons. They are preferentially innervated by transient central cells that express eGFP in a GRP-eGFP mouse line. Around 40% of projection neurons in lamina I express Tac1, the gene for substance P. Silencing Tac1 cells in the dorsal horn reduces reflex responses to cold and radiant heat. The tachykinin peptide substance P (SP) is expressed by many interneurons and some projection neurons in the superficial dorsal horn of the spinal cord. We have recently shown that SP-expressing excitatory interneurons in lamina II correspond largely to a morphological class known as radial cells. However, little is known about their function, or their synaptic connectivity. Here we use a modification of the Brainbow technique to define the excitatory synaptic input to SP radial cells. We show that around half of their excitatory synapses (identified by expression of Homer) are from boutons with VGLUT2, which are likely to originate mainly from local interneurons. The remaining synapses presumably include primary afferents, which generally have very low levels of VGLUT2. Our results also suggest that the SP cells are preferentially innervated by a population of excitatory interneurons defined by expression of green fluorescent protein under control of the gene for gastrin-releasing peptide, and that they receive sparser input from other types of excitatory interneuron. We show that around 40% of lamina I projection neurons express Tac1, the gene encoding substance P. Finally, we show that silencing Tac1-expressing cells in the dorsal horn results in a significant reduction in reflex responses to cold and radiant heat, but does not affect withdrawal to von Frey hairs, or chloroquine-evoked itch.
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影响因子：
7.4
作者：
Cameron D;Polgár E;Gutierrez-Mecinas M;Gomez-Lima M;Watanabe M;Todd AJ
通讯作者：
Todd AJ
影响因子：
64.5
作者：
Duan B;Cheng L;Bourane S;Britz O;Padilla C;Garcia-Campmany L;Krashes M;Knowlton W;Velasquez T;Ren X;Ross S;Lowell BB;Wang Y;Goulding M;Ma Q
通讯作者：
Ma Q
影响因子：
16.2
作者：
Foster, Edmund;Wildner, Hendrik;Tudeau, Laetitia;Haueter, Sabine;Ralvenius, William T.;Jegen, Monika;Johannssen, Helge;Hoesli, Ladina;Haenraets, Karen;Ghanem, Alexander;Conzelmann, Karl-Klaus;Boesl, Michael;Zeilhofer, Hanns Ulrich
通讯作者：
Zeilhofer, Hanns Ulrich
影响因子：
48
作者：
Cai D;Cohen KB;Luo T;Lichtman JW;Sanes JR
通讯作者：
Sanes JR
影响因子：
5.5
作者：
Grudt, TJ;Perl, ER
通讯作者：
Perl, ER