The Turkey Ig-like receptor family: identification, expression and function.
The Turkey Ig-like receptor family: identification, expression and function.
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DOI：
10.1371/journal.pone.0059577
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Göbel TW
中科院分区：
文献类型：
--
作者：
Windau K;Viertlboeck BC;Göbel TW
鸡白细胞受体复合物位于31号染色体上，编码鸡Ig样受体（CHIR），这是一个广泛扩展的基因家族，可进一步分为三个亚群：激活性CHIR-A、双功能CHIR-AB和抑制性CHIR-B。在这里，我们调查了其他鸟类中CHIR同系物的存在。利用不同的策略对火鸡、鸭和斑胸草雀的基因组数据库进行了筛选，包括使用不同CHIR序列的BLAST检索和关键词检索。我们不能确定在远亲斑马雀和鸭的CHIR同源物，然而，几个部分和完整的CHIR同源物序列被确定在3号染色体上的火鸡基因组。它们被命名为火鸡免疫球蛋白样受体（TILR）。从火鸡血和脾RNA中扩增出6个TILR基因，根据其序列特征可将其分为1个激活型TILR-A、1个抑制型TILR-B和4个双功能TILR-AB。由于TILR-AB序列都显示出参与与IgY结合的关键残基，我们接下来使用可溶性TILR-AB 1-huIg融合蛋白证实了IgY结合。该融合蛋白能与多种鸡源性IgY发生反应，但不与其他鸟类IgY发生反应。最后，我们测试了针对CHIR的各种mAb与火鸡或鸭白细胞的交叉反应性。虽然鸭细胞未检测到染色，但CHIR-AB 1特异性mAb 8D 12和CHIR-A2特异性mAb 13 E2均与白细胞亚群反应，通过使用B细胞、T细胞和血小板特异性试剂的双重免疫荧光进一步将其鉴定为血小板。总之，虽然火鸡与鸡具有相似的LRC基因，但它们的分布似乎不同，主要分布在血小板上而不是淋巴细胞上。
The chicken leukocyte receptor complex located on microchromosome 31 encodes the chicken Ig-like receptors (CHIR), a vastly expanded gene family which can be further divided into three subgroups: activating CHIR-A, bifunctional CHIR-AB and inhibitory CHIR-B. Here, we investigated the presence of CHIR homologues in other bird species. The available genome databases of turkey, duck and zebra finch were screened with different strategies including BLAST searches employing various CHIR sequences, and keyword searches. We could not identify CHIR homologues in the distantly related zebra finch and duck, however, several partial and complete sequences of CHIR homologues were identified on chromosome 3 of the turkey genome. They were designated as turkey Ig-like receptors (TILR). Using cDNA derived from turkey blood and spleen RNA, six full length TILR could be amplified and further divided according to the typical sequence features into one activating TILR-A, one inhibitory TILR-B and four bifunctional TILR-AB. Since the TILR-AB sequences all displayed the critical residues shown to be involved in binding to IgY, we next confirmed the IgY binding using a soluble TILR-AB1-huIg fusion protein. This fusion protein reacted with IgY derived from various gallinaceous birds, but not with IgY from other bird species. Finally, we tested various mab directed against CHIR for their crossreactivity with either turkey or duck leukocytes. Whereas no staining was detectable with duck cells, the CHIR-AB1 specific mab 8D12 and the CHIR-A2 specific mab 13E2 both reacted with a leukocyte subpopulation that was further identified as thrombocytes by double immunofluorescence employing B-cell, T-cell and thrombocyte specific reagents. In summary, although the turkey harbors similar LRC genes as the chicken, their distribution seems to be distinct with predominance on thrombocytes rather than lymphocytes.
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