Molecular identification and prevalence of trypanosomes in cattle distributed within the Jebba axis of the River Niger, Kwara state, Nigeria.
Molecular identification and prevalence of trypanosomes in cattle distributed within the Jebba axis of the River Niger, Kwara state, Nigeria.
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分布在尼日利亚夸拉州尼日尔河热巴轴线内的牛体内锥虫的分子鉴定和患病率。
DOI：
10.1186/s13071-021-05054-0
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发表时间：
2021-10-29
影响因子：
3.2
通讯作者：
Kwaga JKP
中科院分区：
文献类型：
--
作者：
Habeeb IF;Chechet GD;Kwaga JKP
锥虫病是一种致命疾病，威胁着撒哈拉以南非洲至少37个国家的经济，特别是畜牧业。在这项研究中，我们调查了牛锥虫感染的患病率，并从分子水平上确定了感染牛的锥虫种类和沿着尼日尔河热巴轴的锥虫感染牛群的空间分布。沿着尼日尔河的热巴轴进行了一项随机横断面研究，采用ITS-1通用引物通过巢式PCR从36个牛群群中筛选牛。使用卡方检验以95%置信区间分析生成的数据。显微镜检查显示，398头受检牛中有3头受感染，患病率为0.8%。12只动物（3.0%）PCR阳性。我们的结果显示，感染动物的红细胞压积下降（24.7%）。感染率分为单一感染11/12（91.7%）和混合感染1/12（8.3%）。动物感染以刚果锥虫最多（50.0%），其次为锥虫。刚果萨凡纳是最常见的亚种（71.4%）。除了按年龄（10.0%）和动物与尼日尔河的相对距离（56.2%）计算的感染率外，其他所有检测参数的统计学差异仅基于概率。空间数据显示，疾病在距离尼日尔河不到3公里的畜群中流行。在尼日尔河热巴轴线沿着牛群中发现6种锥虫，其中T.刚果语是最普遍的。年龄和牛群与尼日尔河的相对距离可能是该地区牛群锥虫感染的危险因素。在线版本包含补充材料，可通过10.1186/s13071-021-05054-0获得。
Trypanosomiasis is a fatal disease that threatens the economy of at least 37 countries in sub-Saharan Africa, particularly with regard to livestock farming. In this study, we investigated the prevalence of trypanosome infection in cattle, and molecularly identified the species of trypanosomes in infected cattle and the spatial distribution of trypanosome-infected herds along the Jebba axis of the River Niger. A randomized cross-sectional study was conducted along the Jebba axis of the River Niger by screening cattle from 36 herd clusters by nested PCR using ITS-1 generic primers. Data generated were analysed using the Chi-square test at a 95% confidence interval. Microscopic examination revealed three infected cattle out of 398 examined, representing 0.8% prevalence. Twelve animals (3.0%) were positive by PCR. Our results showed a decline in the packed cell volume of infected animals (24.7%). The infection rates were categorized as single infection in 11/12 (91.7%) and mixed infection in 1/12 (8.3%). Animals were most frequently infected by Trypanosoma congolense (50.0%), with T. congolense Savannah being the most prevalent subspecies (71.4%). Aside from the infection rate by age (10.0%) and relative distance of animals from the River Niger (56.2%), statistical differences in every other parameter tested were based on mere probabilistic chance. Spatial data showed that the disease was prevalent among herds located less than 3 km from the River Niger. Six species of trypanosomes were identified in cattle herds along the Jebba axis of the River Niger, with T. congolense being the most prevalent. Age and relative distance of herds from the River Niger may be risk factors for trypanosome infection in cattle herds in this area. The online version contains supplementary material available at 10.1186/s13071-021-05054-0.
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DOI：
10.1186/1475-9292-2-8
发表时间：
2003-10-28
期刊：
Kinetoplastid Biology and Disease
影响因子：
--
作者：
Gonzales, Jose Luis;Jones, Tudor W.;Ribera Cuellar, Hugo
通讯作者：
Ribera Cuellar, Hugo
影响因子：
2.7
作者：
de Gier, Jill;Cecchi, Giuliano;Zhao, Weining
通讯作者：
Zhao, Weining
影响因子：
2.7
作者：
Adams, E. R.;Malele, I. I.;Gibson, W. C.
通讯作者：
Gibson, W. C.
影响因子：
2.6
作者：
Herrera, HM;Dávila, AMR;Jansen, AM
通讯作者：
Jansen, AM
影响因子：
--
作者：
Garcia, H.;Garcia, M. -E.;Mendoza-Leon, A.
通讯作者：
Mendoza-Leon, A.