Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn.
Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn.
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DOI：
10.1097/j.pain.0000000000002194
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发表时间：
2021-07-01
期刊：
影响因子：
7.4
通讯作者：
Graham BA
中科院分区：
文献类型：
--
作者：
Browne TJ;Smith KM;Gradwell MA;Iredale JA;Dayas CV;Callister RJ;Hughes DI;Graham BA
文本中提供了补充数字内容。小鼠 I 层脊髓旁臂投射神经元表现出异质的电生理学和形态学特征，可用于区分 4 个离散的亚群。层 III-V 脊髓旁臂投射神经元也表现出定义的电生理特性。脊髓背角的投射神经元将感觉信息传递到更高的大脑中枢。这些群体的激活是由来自外周的传入输入、来自大脑的下行输入和来自局部中间神经元回路的输入决定的。我们最近对背角电路的了解大部分来自对转基因小鼠的研究。然而，关于投射神经元的信息仍然主要基于对猴子、猫和大鼠的研究。我们使用病毒标记来识别和记录位于脊髓切片背角的小鼠臂旁核（PBN）投射神经元。总体而言，小鼠 I 层脊髓旁臂投射神经元 (SPBN) 表现出许多与大鼠重叠的电生理学和形态学特征。无偏聚类分析根据 I SPBN 层的 4 个不同亚群的电生理学特性区分了它们，这些电生理学特性可能是每组中不同感觉信号特征的基础。我们还提供了有关更深层（III-V）中 SPBN 的新信息，这些信息以前尚未通过膜片钳分析进行研究。与层板 I SPBN 相比，这些神经元表现出更高的动作电位放电频率，并且接收到的兴奋性突触输入更弱，这表明这个更深的群体产生了针对 PBN 的不同感觉代码。来自这两个区域（层 I 和 III-V）的小鼠 SPBN 通常会发出局部轴突侧支，我们提供了神经解剖学证据，它们有助于背角回路的兴奋性输入。这些数据提供了新的信息来暗示在处理伤害性信息期间背角电路活动中臂旁投射神经元的兴奋性输入，以及定义深部背角投射神经元，其提供了感觉信息到达 PBN 的替代途径。
Supplemental Digital Content is Available in the Text. Mouse lamina I spinoparabrachial projection neurons exhibit heterogenous electrophysiological and morphological characteristics that can be used to distinguish 4 discrete subpopulations. Lamina III-V spinoparabrachial projection neurons also exhibit defining electrophysiological properties. Projection neurons in the spinal dorsal horn relay sensory information to higher brain centres. The activation of these populations is shaped by afferent input from the periphery, descending input from the brain, and input from local interneuron circuits. Much of our recent understanding of dorsal horn circuitry comes from studies in transgenic mice; however, information on projection neurons is still based largely on studies in monkey, cat, and rat. We used viral labelling to identify and record from mouse parabrachial nucleus (PBN) projecting neurons located in the dorsal horn of spinal cord slices. Overall, mouse lamina I spinoparabrachial projection neurons (SPBNs) exhibit many electrophysiological and morphological features that overlap with rat. Unbiased cluster analysis distinguished 4 distinct subpopulations of lamina I SPBNs, based on their electrophysiological properties that may underlie different sensory signalling features in each group. We also provide novel information on SPBNs in the deeper lamina (III-V), which have not been previously studied by patch clamp analysis. These neurons exhibited higher action potential discharge frequencies and received weaker excitatory synaptic input than lamina I SPBNs, suggesting this deeper population produces different sensory codes destined for the PBN. Mouse SPBNs from both regions (laminae I and III-V) were often seen to give off local axon collaterals, and we provide neuroanatomical evidence they contribute to excitatory input to dorsal horn circuits. These data provide novel information to implicate excitatory input from parabrachial projection neuron in dorsal horn circuit activity during processing of nociceptive information, as well as defining deep dorsal horn projection neurons that provide an alternative route by which sensory information can reach the PBN.
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影响因子：
7.4
作者：
Cameron D;Polgár E;Gutierrez-Mecinas M;Gomez-Lima M;Watanabe M;Todd AJ
通讯作者：
Todd AJ
DOI：
10.1126/science.aac8653
发表时间：
2015-10-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bourane S;Duan B;Koch SC;Dalet A;Britz O;Garcia-Campmany L;Kim E;Cheng L;Ghosh A;Ma Q;Goulding M
通讯作者：
Goulding M
影响因子：
64.5
作者：
Duan B;Cheng L;Bourane S;Britz O;Padilla C;Garcia-Campmany L;Krashes M;Knowlton W;Velasquez T;Ren X;Ross S;Lowell BB;Wang Y;Goulding M;Ma Q
通讯作者：
Ma Q
影响因子：
3.3
作者：
Al Ghamdi, K. S.;Polgar, E.;Todd, A. J.
通讯作者：
Todd, A. J.
影响因子：
16.2
作者：
Foster, Edmund;Wildner, Hendrik;Tudeau, Laetitia;Haueter, Sabine;Ralvenius, William T.;Jegen, Monika;Johannssen, Helge;Hoesli, Ladina;Haenraets, Karen;Ghanem, Alexander;Conzelmann, Karl-Klaus;Boesl, Michael;Zeilhofer, Hanns Ulrich
通讯作者：
Zeilhofer, Hanns Ulrich