Centrioles are amplified in cycling progenitors of olfactory sensory neurons.
Centrioles are amplified in cycling progenitors of olfactory sensory neurons.
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DOI：
10.1371/journal.pbio.3000852
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发表时间：
2020-09
期刊：
影响因子：
9.8
通讯作者：
Stearns T
中科院分区：
文献类型：
--
作者：
Ching K;Stearns T
大多数动物的嗅觉是由带有纤毛的神经元调节的，这些纤毛是环境中可以接触到的。脊索动物的嗅觉神经元通常有多个纤毛，每个纤毛的底部都有一个中心粒。嗅鞘细胞是由嗅觉上皮中的干细胞分化而来的，而嗅觉上皮如何产生具有许多中心粒的细胞尚不清楚。我们发现，在成年小鼠的胚胎发育和嗅觉上皮的更替过程中，中心粒都是通过中心粒玫瑰花环的形成而被放大的，而且玫瑰花环的细胞通常还有游离的中心粒。中心粒放大的细胞可以继续分裂，中心粒聚集在每一极。此外，我们还发现，在即刻神经元前体(INPs)中，中心粒被放大，同时伴随着Polo-like kinase4(Plk4)和SCL/Tal1-interrupting Locus gene(STIL)的mRNA的上调，这两个基因是中心粒复制的关键调节因素。这些结果支持中心粒放大发生在以早期INP细胞Plk4和STIL升高为特征的瞬时状态的模型。然后这些细胞至少分裂一次，成为OSN，这表明带有放大的中心粒的细胞分裂可以发生，这是已知在疾病状态下可以容忍的，作为正常发育程序的一部分。嗅觉神经元(OSN)在突入鼻腔的树突末端有大约15根纤毛。这项研究表明，这些神经元的祖细胞放大他们的中心粒，然后进行一次或多次分裂成为OSNs；这伴随着驱动中心粒形成的蛋白质表达的一过性增加。
Olfaction in most animals is mediated by neurons bearing cilia that are accessible to the environment. Olfactory sensory neurons (OSNs) in chordates usually have multiple cilia, each with a centriole at its base. OSNs differentiate from stem cells in the olfactory epithelium, and how the epithelium generates cells with many centrioles is not yet understood. We show that centrioles are amplified via centriole rosette formation in both embryonic development and turnover of the olfactory epithelium in adult mice, and rosette-bearing cells often have free centrioles in addition. Cells with amplified centrioles can go on to divide, with centrioles clustered at each pole. Additionally, we found that centrioles are amplified in immediate neuronal precursors (INPs) concomitant with elevation of mRNA for polo-like kinase 4 (Plk4) and SCL/Tal1-interrupting locus gene (Stil), key regulators of centriole duplication. These results support a model in which centriole amplification occurs during a transient state characterized by elevated Plk4 and Stil in early INP cells. These cells then go on to divide at least once to become OSNs, demonstrating that cell division with amplified centrioles, known to be tolerated in disease states, can occur as part of a normal developmental program. Olfactory sensory neurons (OSNs) have about fifteen cilia at the end of a dendrite that projects into the nasal cavity. This study shows that the progenitors of these neurons amplify their centrioles and then proceed to divide one or more times to become OSNs; this is accompanied by a transient increase in the expression of proteins that drive centriole formation.
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影响因子：
10.5
作者：
Ma L;Quigley I;Omran H;Kintner C
通讯作者：
Kintner C
影响因子：
3.7
作者：
Silkworth WT;Nardi IK;Scholl LM;Cimini D
通讯作者：
Cimini D
影响因子：
64.8
作者：
Al Jord, Adel;Lemaitre, Anne-Iris;Meunier, Alice
通讯作者：
Meunier, Alice
影响因子：
21.3
作者：
Habedanck, R;Stierhof, YD;Nigg, EA
通讯作者：
Nigg, EA
DOI：
10.1083/jcb.10.2.163
发表时间：
1961-06
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
GALL, J G
通讯作者：
GALL, J G