Organization and evolutionary progress of a dispersed repetitive family of sequences in widely separated rodent genomes.
Organization and evolutionary progress of a dispersed repetitive family of sequences in widely separated rodent genomes.
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广泛分离的啮齿动物基因组中分散的重复序列家族的组织和进化进展。
DOI：
10.1016/0022-2836(81)90374-0
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发表时间：
1981
影响因子：
5.6
通讯作者：
G. Dover
中科院分区：
文献类型：
--
作者：
S. Brown;G. Dover
小家鼠和其他啮齿动物物种的基因组共享一个长期保守的序列家族，这些序列是分散的和丰富的（约1000个）。20，000个拷贝），并具有一些新的组织和进化特征。musculusDNA显示了一组显著的1350 bp的序列。850和500 bp的两个非同源序列，代表几乎总人口的1350 bp的重复序列，被用来检查的详细组织的分散的家庭及其周围的序列，使用限制性内切酶分析和“Southern”杂交的组合。1350 bp序列包含在约3 kb的较长重复单元内，该重复单元分散在各种各样的非同源和看似非重复的序列中。在3 kb重复序列内的某些位点，在该家族成员之间发现了相当大的序列异质性，使得该家族可以根据Hin III位点的定位被分成很大程度上不重叠的子集（或“片段”）。在片段组织的基础上，每个片段与每个其他片段的背景重叠很低。在中国仓鼠M. musculus，X染色体细胞系：表明该家族基因组分布广泛。同源重复序列的M。在家蝇和姬鼠中发现了一个1350 bp的重复序列，它们在组织和分散特征上具有惊人的相似性。InM. spretusa 1350 bp序列包含在至少2.9 kb的分散重复序列中。然而，大多数M。spretusrepeats含有一个额外的限制性位点，不存在于等价物M中。森林姬鼠有两个分散的、同源的家族，分别为1350 bp和1850 bp的重复序列，其中含有从mM分化出来的序列。musculus to different extends. Mystacinus只有一个1850 bp的分散同源重复序列家族。大多数成员在每个Apodemushomaltus家庭也包含特征性的变异限制位点的安排。这种变异在每个阵列内的传播的机制;节段模式的产生;和这种小鼠散布的家庭（MIF-1）的进化保护进行了讨论，与目前的知识的组织和活动的其他分散的序列家庭。
The genomes ofMus musculusand other rodent species share a long conserved family of sequences that are dispersed and abundant (approx. 20,000 copies), and that have several novel features of organization and evolution.EcoR1 restriction ofM. musculusDNA reveals a prominent 1350 bp†set of sequences. Two nonhomologous sequences of 850 and 500 bp, representing almost the total population of the 1350 bp repeats, were used to examine the detailed organization of the dispersed family and its surrounding sequences using a combination of restriction analysis and “Southern” hybridization. The 1350 bp sequence is contained within a longer repeating unit of approximately 3 kb that is dispersed amongst a wide variety of non-homologous and seemingly non-repetitive sequences. At some sites within the 3 kb repeat, considerable sequence heterogeneity has been found between members of the family, such that the family can be divided into largely non-overlapping subsets (or “segments”) according to the positioning ofHinIII sites. Underlying the segmental organization there is a low background overlap of each segment with every other. Some but not all members of the family and its variants have been located on the X-chromosome in a Chinese hamster,M. musculus, X chromosome cell line: suggesting a wide genomic dispersion of the family. Homologous repeated sequences to theM. musculus1350 bp repeat have been identified in species ofMusandApodemus, with strikingly similar features of organization and dispersion. InM. spretusa 1350 bp sequence is contained within a dispersed repeat of at least 2·9 kb. However, the majority ofM. spretusrepeats contain an additional restriction site not present in the equivalentM. musculusarray, suggesting a mechanism of widespread substitution or “conversion” of one variant by another in each genome.Apodemus sylvaticuspossesses two dispersed and homologous families of 1350 bp and 1850 bp repetition, respectively, which contain sequences that have diverged fromM. musculusto differing extents.A. mystacinuspossesses only one family of dispersed and homologous repeats of 1850 bp. The majority of members within eachApodemushomologous family also contain characteristic variant restriction-site arrangements. The mechanisms underlying the spread of such variants within each array; the generation of segmental patterns; and the evolutionary conservation of this mouse interspersed family (MIF-1) are discussed in relation to the present knowledge of the organization and activity of other dispersed sequence families.
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作者：
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DOI：
10.1126/science.6251545
发表时间：
1980
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Scherer,S;Davis,RW
通讯作者：
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