Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
Suppression of activation and induction of apoptosis in RAW264.7 cells by amniotic membrane extract.
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DOI：
10.1167/iovs.08-1781
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发表时间：
2008-10
影响因子：
4.4
通讯作者：
Tseng, Scheffer C. G.
中科院分区：
文献类型：
--
作者：
He, Hua;Li, Wei;Chen, Szu-Yu;Zhang, Shan;Chen, Ying-Ting;Hayashida, Yasutaka;Zhu, Ying-Ting;Tseng, Scheffer C. G.
巨噬细胞在启动、维持和解决宿主炎症/免疫反应中起着关键作用，但如果不加以控制，可能导致顽固性炎症和组织损伤。临床上，羊膜移植可抑制眼表重建中的炎症反应。实验中，作者和其他人报道了AM促进巨噬细胞凋亡。然而，AM提取物（AME）是否保留这种抗炎活性尚不清楚。作者在静息和激活（干扰素[IFN]-γ，脂多糖[LPS]或IFN-γ/LPS）小鼠单核/巨噬细胞RAW264.7细胞中证明，AME抑制细胞扩散并减少肌动蛋白丝，通过phalloidin染色和Triton X-100提取细胞裂解物的Western blotting测定。Western blot和免疫细胞化学染色显示AME下调CD80、CD86和主要组织相容性复合体2类抗原等细胞表面标志物的表达。AME可抑制细胞生长/活力，增强细胞凋亡。因此，分泌的促炎细胞因子如TNF-α和IL-6减少，但抗炎细胞因子IL-10上调。综上所述，这些结果表明，与羊膜类似，AME通过下调巨噬细胞的活化和诱导细胞凋亡来保持抗炎活性。
Macrophages play a pivotal role in initiating, maintaining, and resolving host inflammatory/immune responses but may cause recalcitrant inflammation and tissue damage if not controlled. Clinically, amniotic membrane (AM) transplantation suppresses inflammation in ocular surface reconstruction. Experimentally, the authors and others have reported that AM facilitates macrophage apoptosis. However, it remains unclear whether such anti-inflammatory activity is retained in AM extract (AME). Herein the authors demonstrate in resting and activated (by interferon [IFN]-γ, lipopolysaccharide [LPS], or IFN-γ/LPS) murine monocyte/macrophage RAW264.7 cells that AME suppresses cell spreading and reduces actin filaments determined by phalloidin staining and Western blotting of Triton X-100 extracted cell lysate. Western blot and immunocytochemistry staining showed AME downregulates the expression of such cell surface markers as CD80, CD86, and major histocompatibility complex class 2 antigen. Cell growth/viability is inhibited whereas cell apoptosis is enhanced by AME. Accordingly, secreted proinflammatory cytokines such as TNF-α and IL-6 are reduced, but anti-inflammatory cytokine IL-10 is upregulated. Collectively, these results suggest that, similar to amniotic membrane, AME retains anti-inflammatory activities and does so by downregulating activation and inducing apoptosis in macrophages.
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影响因子：
5.5
作者：
Lasunskaia, Elena B.;Campos, Mariana N. N.;Da Silva, Wilmar D.
通讯作者：
Da Silva, Wilmar D.
影响因子：
56.9
作者：
Randolph, GJ;Beaulieu, S;Muller, WA
通讯作者：
Muller, WA
DOI：
10.1016/0167-5699(94)90150-3
发表时间：
1994-03-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
CELADA, A;NATHAN, C
通讯作者：
NATHAN, C
影响因子：
3.4
作者：
Li, W;He, H;Tseng, SCG
通讯作者：
Tseng, SCG
影响因子：
4.4
作者：
Ripoll, Vera M.;Irvine, Katharine M.;Hume, David A.
通讯作者：
Hume, David A.