Chiral magnetic microspheres purified by centrifugal field flow fractionation and microspheres magnetic chiral chromatography for benzoin racemate separation.
Chiral magnetic microspheres purified by centrifugal field flow fractionation and microspheres magnetic chiral chromatography for benzoin racemate separation.
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DOI：
10.1016/j.chroma.2013.07.044
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发表时间：
2013-08-30
期刊：
影响因子：
--
通讯作者：
Wei Y
中科院分区：
文献类型：
--
作者：
Tian A;Qi J;Liu Y;Wang F;Ito Y;Wei Y
由于对映体在手性环境中具有相同的物理和化学性质，对映体的分离仍然是一个挑战，并且对特定手性选择剂和分离技术的研究仍在继续进行，以解析单个对映体。我们实验室开发了一种很有前途的磁性手性微球Fe3O4@SiO2@纤维素-2, 3-双(3, 5-二甲基苯基氨基甲酸酯)，利用其磁性和手性识别能力促进拆分。在我们目前的研究中，这种磁性手性选择剂首先通过离心场流分级纯化，然后通过色谱方法用于分离安息香外消旋体。首先通过安装在 J 型行星离心机上的螺旋柱与乙醇进行场流分馏，获得尺寸均匀且已去除掩蔽杂质的磁性手性选择剂，并使用纯化的磁性手性选择剂，通过浸没在干冰中的盘管（缠绕在圆柱形磁体上，以将磁性手性选择剂保留为固定相）用乙醇洗脱，成功地完成安息香外消旋体的最终色谱分离。此外，外部磁场有利于磁性手性选择器的回收。
Separation of enantiomers still remains a challenge due to their identical physical and chemical properties in a chiral environment, and the research on specific chiral selector along with separation techniques continues to be conducted to resolve individual enantiomers. In our laboratory the promising magnetic chiral microspheres Fe3O4@SiO2@cellulose-2, 3-bis (3, 5-dimethylphenylcarbamate) have been developed to facilitate the resolution using both its magnetic property and chiral recognition ability. In our present studies this magnetic chiral selector was first purified by centrifuge field flow fractionation, and then used to separate benzoin racemate by a chromatographic method. Uniform-sized and masking-impurity-removed magnetic chiral selector was first obtained by field flow fractionation with ethanol through a spiral column mounted on the type-J planetary centrifuge, and using the purified magnetic chiral selector, the final chromatographic separation of benzoin racemate was successfully performed by eluting with ethanol through a coiled tube (wound around the cylindrical magnet to retain the magnetic chiral selector as a stationary phase) submerged in dry ice. In addition, an external magnetic field facilitates the recycling of the magnetic chiral selector.
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
7.4
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影响因子：
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作者：
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