Age-related differences in the neutrophil response to pulmonary pseudomonas infection.
Age-related differences in the neutrophil response to pulmonary pseudomonas infection.
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DOI：
10.1016/j.exger.2013.12.010
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发表时间：
2014-06
影响因子：
3.9
通讯作者：
Kovacs, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Chen, Michael M.;Palmer, Jessica L.;Plackett, Timothy P.;Deburghgraeve, Cory R.;Kovacs, Elizabeth J.
铜绿假单胞菌肺炎在老年人中更常见，也更致命。这种发病率和死亡率增加的免疫学基础尚未得到评估，但被认为是年龄相关性免疫功能障碍的并发症。用幼龄(10~12周龄)和老年(18~20月龄)BALB/c小鼠气管内感染铜绿假单胞菌。接种后24小时处死动物。收集肺进行肺病理、趋化因子水平、中性粒细胞计数和髓过氧化物酶活性分析。感染铜绿假单胞菌后，老年小鼠肺中中性粒细胞趋化因子KC的水平明显高于年轻小鼠。尽管如此，感染后年轻小鼠的中性粒细胞计数比老年小鼠高。此外，中性粒细胞主要存在于感染幼鼠的空气中。这与感染后幼鼠的支气管肺泡灌洗液标本中髓过氧化物酶活性高于老龄鼠有关。尽管趋化因子水平升高，但在气管内感染后24小时，老年小鼠的中性粒细胞向肺部的迁移受到损害，这表明免疫衰老正在损害中性粒细胞的迁移。
Pseudomonas aeruginosa pneumonia is more common and more lethal in the elderly. The immunologic underpinnings of this increased incidence and mortality has not been evaluated, however is assumed to be a complication of age-associated immune dysfunction. Young (10–12 week old) and aged (18–20 month old) BALB/c mice were subjected to intratracheal infection of Pseudomonas aeruginosa. Animals were sacrificed 24 hours after inoculation. Lungs were collected for analysis of lung pathology, chemokine levels, neutrophil counts, and myeloperoxidase activity. Pulmonary levels of the neutrophil chemokine KC are significantly higher in aged mice relative to young following P. aeruginosa infection. Despite this, neutrophil counts are higher in young mice compared to aged mice after infection. Furthermore, the neutrophils are predominantly found in the air space of young infected mice. This correlated with increased myeloperoxidase activity from bronchoalveolar lavage specimens of young mice relative to aged mice after infection. Neutrophil migration into the lungs is impaired in aged mice 24 hours after intratracheal infection despite elevated chemokine levels, suggesting that immunosenescence is impairing neutrophil migration.
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DOI：
10.4049/jimmunol.1201213
发表时间：
2013-02-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Brubaker AL;Rendon JL;Ramirez L;Choudhry MA;Kovacs EJ
通讯作者：
Kovacs EJ
影响因子：
8.8
作者：
McConnell, Kevin W.;McDunn, Jonathan E.;Coppersmith, Craig M.
通讯作者：
Coppersmith, Craig M.
影响因子：
38.9
作者：
Tumbarello, Mario;De Pascale, Gennaro;Antonelli, Massimo
通讯作者：
Antonelli, Massimo
DOI：
10.1165/ajrcmb.20.6.3491
发表时间：
1999-06-01
影响因子：
6.4
作者：
Patel, PJ;Faunce, DE;Kovacs, EJ
通讯作者：
Kovacs, EJ
影响因子：
8.8
作者：
Gomez, Christian R.;Hirano, Stefanie;Kovacs, Elizabeth J.
通讯作者：
Kovacs, Elizabeth J.