Intracameral Microimaging of Maturation of Human iPSC Derivatives into Islet Endocrine Cells.
Intracameral Microimaging of Maturation of Human iPSC Derivatives into Islet Endocrine Cells.
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DOI：
10.1177/09636897211066508
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发表时间：
2022-01
影响因子：
3.3
通讯作者：
Yang SN
中科院分区：
文献类型：
--
作者：
Zhao K;Shi Y;Yu J;Yu L;Mael A;Li Y;Kolton A;Joyce T;Odorico J;Berggren PO;Yang SN
我们利用免疫缺陷小鼠的眼前房（ACE）作为移植和显微成像来自人诱导多能干细胞（hiPSC-SI）的工程替代胰岛的异位部位。这些胰岛含有大部分表达胰岛素的细胞（PDX 1和NKX6.1阳性或阴性）以及少数胰高血糖素或生长激素抑制素阳性细胞。单个非聚集的hiPSC-SI令人满意地移植到虹膜上。它们经历了逐渐的血管化并逐渐增加其光散射信号，反映了包装在成熟胰岛素分泌颗粒内的锌胰岛素晶体的丰度。从受体中取出的前房内hiPSC-SI显示出增强的胰岛素免疫荧光，与移植后期间总体血管化和光背散射的平行增加相关。该方法能够对单个hiPSC-SI移植物的细胞命运、植入、血管化和成熟胰岛素分泌颗粒进行纵向、非破坏性和活体显微成像，并且可以提供可行且可靠的手段来筛选用于促进体内hiPSC-SI成熟的化合物。
We exploited the anterior chamber of the eye (ACE) of immunodeficient mice as an ectopic site for both transplantation and microimaging of engineered surrogate islets from human induced pluripotent stem cells (hiPSC-SIs). These islets contained a majority of insulin-expressing cells, positive or negative for PDX1 and NKX6.1, and a minority of glucagon- or somatostatin-positive cells. Single, non-aggregated hiPSC-SIs were satisfactorily engrafted onto the iris. They underwent gradual vascularization and progressively increased their light scattering signals, reflecting the abundance of zinc-insulin crystal packaged inside mature insulin secretory granules. Intracameral hiPSC-SIs retrieved from recipients showed enhanced insulin immunofluorescence in correlation with the parallel increase in overall vascularization and light backscattering during the post-transplantation period. This approach enables longitudinal, nondestructive and intravital microimaging of cell fates, engraftment, vascularization and mature insulin secretory granules of single hiPSC-SI grafts, and may offer a feasible and reliable means to screen compounds for promoting in vivo hiPSC-SI maturation.
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影响因子：
16.6
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
1954-01-01
期刊：
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1105002108
发表时间：
2011-08-02
影响因子：
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作者：
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通讯作者：
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