Collagen synthesis by human skin fibroblasts in culture: studies of fibroblasts explanted from papillary and reticular dermis.

Collagen synthesis by human skin fibroblasts in culture: studies of fibroblasts explanted from papillary and reticular dermis.
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培养中人皮肤成纤维细胞的胶原合成:从乳头状和网状真皮中移植的成纤维细胞的研究。

DOI:
10.1111/1523-1747.ep12494614
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发表时间:
1981
期刊:
The Journal of investigative dermatology
影响因子:
--
通讯作者:
Pinnell,SR
Pinnell,SR
中科院分区:
--
文献类型:
--
作者:
Tajima,S;Pinnell,SR

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从乳头状和网状真皮建立匹配的人皮肤成纤维细胞培养物。与其匹配的网状真皮培养物相比,乳头状真皮成纤维细胞表现出增加的铺板效率、指数生长和汇合密度。然而,这些细胞的胶原蛋白合成基本上是相似的,无论它们的来源如何。相对胶原合成在汇合密度下是相似的。类型特异性胶原蛋白的合成没有差异,可以检测到的相对量的I型和III型胶原蛋白的培养基和I型,III型和AB型胶原蛋白的细胞团块是相似的。与乳头状真皮成纤维细胞相比,网状真皮成纤维细胞的培养基中I型pC胶原蛋白始终升高。这种差异在前胶原加工中的意义尚不清楚。
Matched human skin fibroblast cultures were established from papillary and reticular dermis. Papillary dermal fibroblasts exhibited increased plating efficiency, exponential growth, and confluent density when compared with their matched reticular dermal cultures. Collagen synthesis by these cells, however, was essentially similar regardless of their origin. Relative collagen synthesis was similar at confluent densities. No differences in type specific collagen synthesis could be detected; relative amounts of types I and III collagens in culture media and types I, III, and AB collagens in the cellular pellet were similar. Type I pC collagen was consistently elevated in culture media from reticular dermal fibroblasts when compared to papillary dermal fibroblasts. The significance of this difference in procollagen processing is unknown.
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影响因子: --
作者:
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