Post-Thaw Storage Temperature Influenced Boar Sperm Quality and Lifespan through Apoptosis and Lipid Peroxidation.
Post-Thaw Storage Temperature Influenced Boar Sperm Quality and Lifespan through Apoptosis and Lipid Peroxidation.
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DOI：
10.3390/ani14010087
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发表时间：
2023-12-26
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由于冷冻对精子质量和生育力的不利影响，冷冻解冻的猪精液尚未被广泛使用。由于猪精子冻融后寿命短，影响了其在田间的应用，因此人们进行了大量的尝试来提高冻融后精子的质量。本研究旨在寻找最佳的解冻后保存方法（保存时间和温度），以延长精子的存活时间，而不影响精子的质量。结果表明，解冻后保存时间和温度对猪精子存活率有影响。解冻后的猪精液在17 °C下保存的精子质量更好，并保持精子质量长达6 h。这证明了在用于人工授精之前，冻融猪精液的保存得到了改善，因为它提供了足够的时间来制备剂量并将其从实验室运输到农场。这些研究结果将有助于推广猪冻精人工授精技术在养猪业中的应用。冷冻保存降低了公猪精子的质量和寿命，限制了猪精液冻融后人工授精在田间条件下的应用。本研究的目的是测试解冻后保存时间和温度对公猪精子存活率的影响。收集来自五头长白猪的精液（每头公猪一次射精），并按照0.5 mL吸管方案冷冻。将来自五头公猪的秸秆解冻并在BTS中以1：1（v：v）稀释。将冻融后的精液样品分成3份，分别在5 °C、17 °C和37 °C下保存6 h。在0.5，2和6小时的存储，精子活力，活力，线粒体膜电位，细胞内活性氧（ROS）水平和凋亡的变化进行了测量。在每个时间点检测了猪精子（SPZ）及其周围环境（SN）中的抗氧化剂和氧化剂水平。结果表明，解冻后的储存时间和温度的显着影响和公猪精子质量（总和前向运动，VCL，活力，顶体完整性），早期和晚期精子凋亡的变化，以及在SPZ和SN的MDA水平的变化的影响。与5 °C和37 °C储存相比，17 °C储存的冻融精液样品显示出更好的精子质量、更少的凋亡水平以及更低的SPZ MDA和SN MDA水平。值得注意的是，在17 °C下解冻后储存将公猪精子寿命延长至6小时，而精子质量没有明显降低。总之，在17 °C下储存冷冻解冻的猪精液可保持精子质量长达6小时，这有利于将冷冻保存的猪精液用于田间人工授精。
Frozen-thawed boar semen has not been widely used due to the deleterious effects of freezing on sperm quality and fertility. Extensive attempts have been made to improve post-thaw sperm quality because the short lifespan of frozen and thawed boar sperm impedes its application in field. The present study aimed to find the best post-thaw storage method (storage time and temperature) to extend sperm survival time without affecting the quality. The results showed that post-thaw storage time and temperature influenced boar sperm survival. Storage of thawed boar semen at 17 °C preserved sperm quality better and maintained sperm quality for up to 6 h. This demonstrated an improvement in the preservation of frozen-thawed boar semen before their use for artificial insemination because it provided enough time to prepare the doses and the transport them from the laboratory to the farm. These findings will help to promote the application of frozen-thawed boar semen doses for artificial insemination in the swine industry. Cryopreservation deteriorates boar sperm quality and lifespan, which restricts the use of artificial insemination with frozen-thawed boar semen in field conditions. The objective of this study was to test the effects of post-thaw storage time and temperature on boar sperm survival. Semen ejaculates from five Landrace boars (one ejaculate per boar) were collected and frozen following a 0.5 mL-straw protocol. Straws from the five boars were thawed and diluted 1:1 (v:v) in BTS. The frozen-thawed semen samples were aliquoted into three parts and respectively stored at 5 °C, 17 °C, and 37 °C for up to 6 h. At 0.5, 2, and 6 h of storage, sperm motility, viability, mitochondrial membrane potential, and intracellular reactive oxygen species (ROS) levels and apoptotic changes were measured. Antioxidant and oxidant levels were tested in boar sperm (SPZ) and their surrounding environment (SN) at each timepoint. The results showed significant effects of post-thaw storage time and temperature and an impact on boar sperm quality (total and progressive motility, VCL, viability, acrosome integrity), early and late sperm apoptotic changes, and changes in MDA levels in SPZ and SN. Compared to storage at 5 °C and 37 °C, frozen-thawed semen samples stored at 17 °C displayed better sperm quality, less apoptotic levels, and lower levels of SPZ MDA and SN MDA. Notably, post-thaw storage at 17 °C extended boar sperm lifespan up to 6 h without obvious reduction in sperm quality. In conclusion, storage of frozen-thawed boar semen at 17 °C preserves sperm quality for up to 6 h, which facilitates the use of cryopreserved boar semen for field artificial insemination.
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影响因子：
3.3
作者：
Balogun，Kayode B.;Nicholls，Griffin;Stewart，Kara R.
通讯作者：
Stewart，Kara R.
影响因子：
3.2
作者：
Barranco, Isabel;Padilla, Lorena;Parrilla, Inmaculada
通讯作者：
Parrilla, Inmaculada
影响因子：
5.1
作者：
He, Wei-hua;Zhai, Xiao-hu;Di, He-shuang
通讯作者：
Di, He-shuang
影响因子：
2.7
作者：
Casas, I.;Althouse, G. C.
通讯作者：
Althouse, G. C.
DOI：
10.3390/ani13203203
发表时间：
2023-10-13
期刊：
Animals : an open access journal from MDPI
影响因子：
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