Recognition of glycoconjugates byHelicobacter pylori: an apparently high-affinity binding of human polyglycosylceramides, a second sialic acid-based specificity
Recognition of glycoconjugates byHelicobacter pylori: an apparently high-affinity binding of human polyglycosylceramides, a second sialic acid-based specificity
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幽门螺杆菌对糖缀合物的识别：人聚糖基神经酰胺的明显高亲和力结合，第二种基于唾液酸的特异性
作者：
H. Miller‐Podraza;M. A. Milh;J. Bergström;K. Karlsson
据报道，幽门螺杆菌以唾液酸依赖性方式凝集红细胞并与各种其他细胞结合。结合被唾液酸乳糖或胎球蛋白和其他唾液酸化糖蛋白抑制。这种特异性显然需要在琼脂上生长细菌，因为我们发现在营养混合物Ham's F12中生长后，这种特异性丧失了。相反，细菌绑定高亲和力和唾液酸依赖的方式，人红细胞的polyglycosylceramides，一个仍然不完全特征组的复杂glycolipides.Bacteria生长在F12培养基代谢标记与35 S-蛋氨酸和分析结合糖脂的薄层色谱图和电泳后的印迹上的糖蛋白，与人红细胞的糖缀合物的焦点。没有结合到简单的神经节苷脂，包括GM 3或sialylparagloboside，或脑神经节苷脂的混合物。与此相反，人红细胞膜的多糖神经酰胺结合在pmol水平。通过弱酸处理、轻度高碘酸盐氧化或唾液酸酶水解消除活性。红细胞蛋白以及一系列的参考糖蛋白没有结合，除了带3，这是弱活性。然而，这种活性是耐高碘酸oxidation.These结果表明，第二个和新的唾液酸识别特异性，这是独立于先前描述的特异性表达。
Helicobacter pylori has been reported to agglutinate erythrocytes and to bind to various other cells in a sialic acid-dependent way. The binding was inhibited by sialyllactose or fetuin and other sialylated glycoproteins. The specificity apparently requires bacterial growth on agar, since we found that it was lost after growth in the nutrient mixture Ham's F12. Instead, the bacteria bound with high affinity and in a sialic acid-dependent way to polyglycosylceramides of human erythrocytes, a still incompletely characterized group of complex glycolipids.Bacteria grown in F12 medium were metabolically labelled with35S-methionine and analysed for binding to glycolipids on thin-layer chromatograms and to glycoproteins on blots after electrophoresis, with human erythrocyte glycoconjugates in focus. There was no binding to simpler gangliosides including GM3 or sialylparagloboside, or to a mixture of brain gangliosides. In contrast, polyglycosylceramides of human erythrocyte membranes bound at a pmol level. The activity was eliminated by mild acid treatment, mild periodate oxidation or sialidase hydrolysis. Erythrocyte proteins as well as a range of reference glycoproteins did not bind, except band 3, which was weakly active. However, this activity was resistant to periodate oxidation.These results indicate a second and novel sialic acid-recognizing specificity which is expressed independently of the previously described specificity.
支链乳糖胺聚糖的结构，是从成人红细胞中分离出来的带 3 的碳水化合物部分。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fukuda,M;Dell,A;Oates,JE;Fukuda,MN
通讯作者：
Fukuda,MN
从十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶的复制品中洗脱糖蛋白。
DOI：
10.1002/elps.1150190214
发表时间：
1998
期刊：
Electrophoresis.
影响因子：
--
作者：
Szewczyk,B;Pilat,Z;Bienkowska-Szewczyk,K;Summers,DF
通讯作者：
Summers,DF