Spontaneous growth hormone (GH) secretion by unstimulated human lymphocytes and the effects of GH-releasing hormone and somatostatin.
Spontaneous growth hormone (GH) secretion by unstimulated human lymphocytes and the effects of GH-releasing hormone and somatostatin.
复制标题
未受刺激的人类淋巴细胞自发生长激素 (GH) 分泌以及 GH 释放激素和生长抑素的作用。
DOI：
10.1210/jcem.79.6.7989474
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
H. Kurahachi
中科院分区：
文献类型：
--
作者：
Naoki Hattori;Katsuji Ikekubo;Takashi Ishihara;K. Moridera;Megumu Hino;H. Kurahachi
我们在5名正常受试者中研究了在没有丝裂原的情况下，淋巴细胞的生长激素分泌是否会自发发生，并像内分泌系统一样受到GHRH和生长抑素的调节。采用标准的Ficoll-Hypaque梯度离心法从肝素化的血液中分离外周血单个核细胞，并在有或没有GHRH、生长抑素类似物（SMS 201-995）、环己亚胺或放线菌素d的情况下孵育7天。所有受试者的GH浓度（5 × 10(5)个细胞/mL）随时间的增加而增加，在第7天达到0.47 +/- 0.18 ng/L。一种蛋白质合成抑制剂（环己亚胺）和RNA合成抑制剂（放线菌素D）完全阻断淋巴细胞的生长激素分泌。未经刺激的人淋巴细胞分泌的免疫反应性GH在抗原性和分子量方面与垂体GH相似。GHRH生理浓度(10（-10)-10(-8)mol/L）和SMS 201-995生理浓度(10（-8)-10(-6)mol/L）对人淋巴细胞自发分泌GH无影响。这些结果表明生长激素是自发合成的，并由未受刺激的人淋巴细胞分泌，并且生长激素在免疫系统中的调节不同于在内分泌系统中的调节。
We investigated in five normal subjects whether the secretion of GH from lymphocytes would occur spontaneously without mitogens and be regulated by GHRH and somatostatin as in the endocrine system. Peripheral blood mononuclear cells were isolated from heparinized blood by the standard Ficoll-Hypaque gradient centrifugation method, and incubated for up to 7 days with or without GHRH, somatostatin analog (SMS 201-995), cycloheximide, or actinomycin D. GH levels in the lyophilized samples were measured by a highly sensitive enzyme immunoassay. GH concentration in culture medium (5 x 10(5) cells/mL) time dependently increased in all subjects, reaching 0.47 +/- 0.18 ng/L at day 7. A protein synthesis inhibitor (cycloheximide) and RNA synthesis inhibitor (actinomycin D) completely blocked GH secretion from lymphocytes. Immunoreactive GH secreted by unstimulated human lymphocytes was similar to pituitary GH in terms of antigenicity and molecular weight. Physiological concentrations of GHRH (10(-10)-10(-8) mol/L) and SMS 201-995 (10(-8)-10(-6) mol/L) had no effects on the spontaneous secretion of GH from human lymphocytes. These results indicate that GH is spontaneously synthesized de novo and secreted from unstimulated human lymphocytes, and that the regulation of GH in the immune system differs from that in the endocrine system.
刀豆球蛋白 A 刺激的小鼠脾细胞产生具有催乳素样生物活性和免疫反应性的因子。
DOI：
10.1016/0006-291x(87)91020-5
发表时间：
1987
影响因子：
3.1
作者：
Montgomery,DW;Zukoski,CF;Shah,GN;Buckley,AR;Pacholczyk,T;Russell,DH
通讯作者：
Russell,DH
外源性生长激素治疗会改变内源性生长激素分泌受损的女性的身体成分并增加自然杀伤细胞的活性。
DOI：
10.1016/0026-0495(87)90236-8
发表时间：
1987
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Crist,DM;Peake,GT;Mackinnon,LT;SibbittJr,WL;Kraner,JC
通讯作者：
Kraner,JC
大鼠生长激素（rGH）释放因子和生长抑素对分散垂体细胞中 rGH 释放和合成的影响。
影响因子：
4.8
作者：
Fukata,J;Diamond,DJ;Martin,JB
通讯作者：
Martin,JB